論文 - 詳細
| RRC ID | 36861 |
|---|---|
| 著者 | Nakanishi K, Kakiguchi K, Yonemura S, Nakano A, Morishima N. |
| タイトル | Transient Ca2+ depletion from the endoplasmic reticulum is critical for skeletal myoblast differentiation. |
| ジャーナル | FASEB J |
| Abstract |
Endoplasmic reticulum (ER) stress is a cellular condition in which unfolded proteins accumulate in the ER because of various but specific causes. Physiologic ER stress occurs transiently during myoblast differentiation, and although its cause remains unknown, it plays a critical role in myofiber formation. To examine the mechanism underlying ER stress, we monitored ER morphology during differentiation of murine myoblasts. Novel ER-derived structures transiently appeared prior to myoblast fusion both in vitro and in vivo. Electron microscopy studies revealed that these structures consisted of pseudoconcentric ER cisternae with narrow lumens. Similar structures specifically formed by pharmacologically induced ER Ca(2+) depletion, and inhibition of ER Ca(2+) efflux channels in differentiating myoblasts considerably suppressed ER-specific deformation and ER stress signaling. Thus, we named the novel structures stress-activated response to Ca(2+) depletion (SARC) bodies. Prior to SARC body formation, stromal interaction molecule 1 (STIM1), an ER Ca(2+) sensor protein, formed ER Ca(2+) depletion-specific clusters. Furthermore, myoblast differentiation manifested by myoblast fusion did not proceed under the same conditions as inhibition of ER Ca(2+) depletion. Altogether, these observations suggest that ER Ca(2+) depletion is a prerequisite for myoblast fusion, causing both physiologic ER stress signaling and SARC body formation. |
| 巻・号 | 29(5) |
| ページ | 2137-49 |
| 公開日 | 2015-5-1 |
| DOI | 10.1096/fj.14-261529 |
| PII | fj.14-261529 |
| PMID | 25678623 |
| MeSH | Animals Blotting, Western Calcium / metabolism* Calcium Channels / metabolism Cell Differentiation* Cell Membrane / metabolism* Cells, Cultured Endoplasmic Reticulum / metabolism* Endoplasmic Reticulum Stress* Immunoenzyme Techniques Mice Myoblasts, Skeletal / cytology* Myoblasts, Skeletal / metabolism Signal Transduction Stromal Interaction Molecule 1 |
| IF | 4.966 |
| 引用数 | 10 |
| WOS 分野 | BIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | C2C12(RCB0987) |