RRC ID 36889
著者 Hashimoto M, Sasaki J, Imazato S.
タイトル Investigation of the cytotoxicity of aluminum oxide nanoparticles and nanowires and their localization in L929 fibroblasts and RAW264 macrophages.
ジャーナル J Biomed Mater Res B Appl Biomater
Abstract The biological responses of aluminum oxide (Al2 O3 ) nanoparticles (NPs) and nanowires (NWs) in cultured fibroblasts (L929) and macrophages (RAW264) were evaluated from their cytotoxicities and micromorphologic properties. Cultured cells were exposed to Al2 O3 NPs (13 nm diameter) and Al2 O3 NWs (2-6 × 200-400 nm). Cytotoxicity and genotoxicity were examined by immunostaining with fluorescence microscopy, and nanomaterial localization was studied by using scanning electron microscopy and transmission electron microscopy. The NPs were cytotoxic and genotoxic, whereas the NWs were not. The scanning electron microscopy images showed that the NPs aggregate more on the cell surface than do the NWs. The transmission electron microscopy images showed that the NPs were internalized into the vesicle and nuclei, for both cell types. In contrast, numerous solid NWs were observed as large aggregates in vesicles, but not in nuclei. Nuclear damage was confirmed by measuring cell viability and by immunostaining for NPs. The chemical changes induced by the NPs in the vesicles or cells may cause cell damage because of their large surface area per volume. The extent of NW entrapment was not sufficient to lower the viability of either cell type.
巻・号 104(2)
ページ 241-52
公開日 2016-2-1
DOI 10.1002/jbm.b.33377
PMID 25715832
MeSH Aluminum Oxide* / adverse effects Aluminum Oxide* / chemistry Aluminum Oxide* / pharmacology Animals Cell Line Cytotoxins / adverse effects Cytotoxins / chemistry Cytotoxins / pharmacology DNA Damage Fibroblasts / metabolism* Fibroblasts / pathology Macrophages / metabolism* Macrophages / pathology Materials Testing* Mice Nanoparticles* / adverse effects Nanoparticles* / chemistry Nanowires* / adverse effects Nanowires* / chemistry
IF 2.831
引用数 8
WOS 分野 ENGINEERING, BIOMEDICAL MATERIALS SCIENCE, BIOMATERIALS
リソース情報
ヒト・動物細胞 RAW 264(RCB0535)