論文 - 詳細
| RRC ID | 37013 |
|---|---|
| 著者 | Gao C, Zhuang X, Cui Y, Fu X, He Y, Zhao Q, Zeng Y, Shen J, Luo M, Jiang L. |
| タイトル | Dual roles of an Arabidopsis ESCRT component FREE1 in regulating vacuolar protein transport and autophagic degradation. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
Protein turnover can be achieved via the lysosome/vacuole and the autophagic degradation pathways. Evidence has accumulated revealing that efficient autophagic degradation requires functional endosomal sorting complex required for transport (ESCRT) machinery. However, the interplay between the ESCRT machinery and the autophagy regulator remains unclear. Here, we show that FYVE domain protein required for endosomal sorting 1 (FREE1), a recently identified plant-specific ESCRT component essential for multivesicular body (MVB) biogenesis and plant growth, plays roles both in vacuolar protein transport and autophagic degradation. FREE1 also regulates vacuole biogenesis in both seeds and vegetative cells of Arabidopsis. Additionally, FREE1 interacts directly with a unique plant autophagy regulator SH3 domain-containing protein2 and associates with the PI3K complex, to regulate the autophagic degradation in plants. Thus, FREE1 plays multiple functional roles in vacuolar protein trafficking and organelle biogenesis as well as in autophagic degradation via a previously unidentified regulatory mechanism of cross-talk between the ESCRT machinery and autophagy process. |
| 巻・号 | 112(6) |
| ページ | 1886-91 |
| 公開日 | 2015-2-10 |
| DOI | 10.1073/pnas.1421271112 |
| PII | 1421271112 |
| PMID | 25624505 |
| PMC | PMC4330779 |
| MeSH | Arabidopsis / genetics Arabidopsis / physiology* Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Autophagy / physiology* Carrier Proteins / genetics Carrier Proteins / metabolism* Endosomal Sorting Complexes Required for Transport / metabolism* Fluorescence Resonance Energy Transfer Microscopy, Confocal Microscopy, Electron, Transmission Multivesicular Bodies / metabolism* Photobleaching Protein Transport / genetics Protein Transport / physiology Vesicular Transport Proteins / genetics Vesicular Transport Proteins / metabolism* |
| IF | 9.412 |
| 引用数 | 95 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pst18264 |