論文 - 詳細
| RRC ID | 3704 |
|---|---|
| 著者 | Yoshida K, Suzuki Y, Honda E, Amemiya K, Nakatani T, Ebina M, Narumi K, Satoh K, Munakata H. |
| タイトル | Leucine-rich repeat region of decorin binds to filamin-A. |
| ジャーナル | Biochimie |
| Abstract |
Decorin is a member of the family of small leucine-rich proteoglycans found in the extracellular matrix and has an important role in promoting fiber formation and in controlling cell proliferation. Here, we have investigated whether the leucine-rich repeat (LRR) region of decorin interacts with proteins from human lung fibroblasts by using a yeast two-hybrid assay. We report that the LRR region of decorin interacts with the cytoskeletal protein, filamin-A (ABP-280), a peripheral cytoplasmic protein. This interaction is dependent on the 288 carboxyl-terminal amino acids of filamin-A, which correspond to repeats 22-24 of its conserved beta-sheet structure. We also show that the recombinant LRR region of decorin binds to filamin-A in vitro, and that the deglycosylated core protein of decorin coprecipitates with filamin-A, whereas intact decorin does not. Together, these results suggest that proteins containing the LRR motif that interact with filamin-A may be present in the cytoplasm or at the plasma membrane. |
| 巻・号 | 84(4) |
| ページ | 303-8 |
| 公開日 | 2002-4-1 |
| DOI | 10.1016/s0300-9084(02)01391-3 |
| PII | S0300908402013913 |
| PMID | 12106908 |
| MeSH | Amino Acid Motifs Amino Acid Sequence Blotting, Western Cells, Cultured Contractile Proteins / chemistry Contractile Proteins / genetics Contractile Proteins / metabolism* DNA, Circular / genetics Decorin Electrophoresis, Polyacrylamide Gel Escherichia coli / genetics Escherichia coli / metabolism Extracellular Matrix Proteins Fibroblasts / metabolism Filamins Glutathione Transferase / metabolism Humans Leucine / chemistry Leucine / metabolism* Lung / cytology Microfilament Proteins / chemistry Microfilament Proteins / genetics Microfilament Proteins / metabolism* Molecular Sequence Data Precipitin Tests Protein Binding Proteoglycans / chemistry Proteoglycans / genetics Proteoglycans / metabolism* Recombinant Fusion Proteins / chemistry Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Repetitive Sequences, Nucleic Acid Sequence Alignment Two-Hybrid System Techniques |
| IF | 3.413 |
| 引用数 | 5 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | WI-38 MRC-5 |