RRC ID 3749
Author Asamizu E, Shimoda Y, Kouchi H, Tabata S, Sato S.
Title A positive regulatory role for LjERF1 in the nodulation process is revealed by systematic analysis of nodule-associated transcription factors of Lotus japonicus.
Journal Plant Physiol.
Abstract We have used reverse genetics to identify genes involved in legume-rhizobium symbiosis in Lotus japonicus. We obtained the sequences of 20 putative transcription factors from previously reported large-scale transcriptome data. The transcription factors were classified according to their DNA binding domains and patterns of expression during the nodulation process. We identified two homologues of Medicago truncatula MtHAP2-1, which encodes a CCAAT-binding protein and has been shown to play a role in nodulation. The functions of the remaining genes in the nodulation process have not been reported. Seven genes were found to encode proteins with AP2-EREBP domains, six of which were similar to proteins that have been implicated in ethylene and/or jasmonic acid signal transduction and defense gene regulation in Arabidopsis (Arabidopsis thaliana). We identified a gene, LjERF1, that is most similar to Arabidopsis ERF1, which is up-regulated by ethylene and jasmonic acid and activates downstream defense genes. LjERF1 showed the same pattern of up-regulation in roots as Arabidopsis ERF1. The nodulation phenotype of roots that overexpressed LjERF1 or inhibited LjERF1 expression using an RNA interference construct indicated that this gene functions as a positive regulator of nodulation. We propose that LjERF1 functions as a key regulator of successful infection of L. japonicus by Mesorhizobium loti.
Volume 147(4)
Pages 2030-40
Published 2008-8
DOI 10.1104/pp.108.118141
PII pp.108.118141
PMID 18567832
PMC PMC2492631
MeSH Base Sequence Cyclopentanes / pharmacology Ethylenes / pharmacology Gene Expression / drug effects Gene Expression Profiling Gene Expression Regulation, Plant / drug effects Lotus / genetics* Lotus / metabolism Lotus / microbiology Medicago truncatula / physiology Molecular Sequence Data Multigene Family Oligonucleotide Array Sequence Analysis Oxylipins / pharmacology Peptide Termination Factors / analysis Peptide Termination Factors / chemistry Peptide Termination Factors / physiology* Phylogeny Plant Proteins / analysis Plant Proteins / chemistry Plant Proteins / physiology* Protein Structure, Tertiary RNA Interference Root Nodules, Plant / genetics Root Nodules, Plant / metabolism Root Nodules, Plant / microbiology Transcription Factors / analysis Transcription Factors / chemistry Transcription Factors / physiology Up-Regulation
IF 6.305
Times Cited 34
Lotus / Glycine Gifu B-129