論文 - 詳細
| RRC ID | 3749 |
|---|---|
| 著者 | Asamizu E, Shimoda Y, Kouchi H, Tabata S, Sato S. |
| タイトル | A positive regulatory role for LjERF1 in the nodulation process is revealed by systematic analysis of nodule-associated transcription factors of Lotus japonicus. |
| ジャーナル | Plant Physiol |
| Abstract |
We have used reverse genetics to identify genes involved in legume-rhizobium symbiosis in Lotus japonicus. We obtained the sequences of 20 putative transcription factors from previously reported large-scale transcriptome data. The transcription factors were classified according to their DNA binding domains and patterns of expression during the nodulation process. We identified two homologues of Medicago truncatula MtHAP2-1, which encodes a CCAAT-binding protein and has been shown to play a role in nodulation. The functions of the remaining genes in the nodulation process have not been reported. Seven genes were found to encode proteins with AP2-EREBP domains, six of which were similar to proteins that have been implicated in ethylene and/or jasmonic acid signal transduction and defense gene regulation in Arabidopsis (Arabidopsis thaliana). We identified a gene, LjERF1, that is most similar to Arabidopsis ERF1, which is up-regulated by ethylene and jasmonic acid and activates downstream defense genes. LjERF1 showed the same pattern of up-regulation in roots as Arabidopsis ERF1. The nodulation phenotype of roots that overexpressed LjERF1 or inhibited LjERF1 expression using an RNA interference construct indicated that this gene functions as a positive regulator of nodulation. We propose that LjERF1 functions as a key regulator of successful infection of L. japonicus by Mesorhizobium loti. |
| 巻・号 | 147(4) |
| ページ | 2030-40 |
| 公開日 | 2008-8-1 |
| DOI | 10.1104/pp.108.118141 |
| PII | pp.108.118141 |
| PMID | 18567832 |
| PMC | PMC2492631 |
| MeSH | Base Sequence Cyclopentanes / pharmacology Ethylenes / pharmacology Gene Expression / drug effects Gene Expression Profiling Gene Expression Regulation, Plant / drug effects Lotus / genetics* Lotus / metabolism Lotus / microbiology Medicago truncatula / physiology Molecular Sequence Data Multigene Family Oligonucleotide Array Sequence Analysis Oxylipins / pharmacology Peptide Termination Factors / analysis Peptide Termination Factors / chemistry Peptide Termination Factors / physiology* Phylogeny Plant Proteins / analysis Plant Proteins / chemistry Plant Proteins / physiology* Protein Structure, Tertiary RNA Interference Root Nodules, Plant / genetics Root Nodules, Plant / metabolism Root Nodules, Plant / microbiology Transcription Factors / analysis Transcription Factors / chemistry Transcription Factors / physiology Up-Regulation |
| IF | 6.902 |
| 引用数 | 48 |
| WOS 分野 | PLANT SCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ミヤコグサ・ダイズ | Gifu B-129 |