RRC ID |
3749
|
著者 |
Asamizu E, Shimoda Y, Kouchi H, Tabata S, Sato S.
|
タイトル |
A positive regulatory role for LjERF1 in the nodulation process is revealed by systematic analysis of nodule-associated transcription factors of Lotus japonicus.
|
ジャーナル |
Plant Physiol
|
Abstract |
We have used reverse genetics to identify genes involved in legume-rhizobium symbiosis in Lotus japonicus. We obtained the sequences of 20 putative transcription factors from previously reported large-scale transcriptome data. The transcription factors were classified according to their DNA binding domains and patterns of expression during the nodulation process. We identified two homologues of Medicago truncatula MtHAP2-1, which encodes a CCAAT-binding protein and has been shown to play a role in nodulation. The functions of the remaining genes in the nodulation process have not been reported. Seven genes were found to encode proteins with AP2-EREBP domains, six of which were similar to proteins that have been implicated in ethylene and/or jasmonic acid signal transduction and defense gene regulation in Arabidopsis (Arabidopsis thaliana). We identified a gene, LjERF1, that is most similar to Arabidopsis ERF1, which is up-regulated by ethylene and jasmonic acid and activates downstream defense genes. LjERF1 showed the same pattern of up-regulation in roots as Arabidopsis ERF1. The nodulation phenotype of roots that overexpressed LjERF1 or inhibited LjERF1 expression using an RNA interference construct indicated that this gene functions as a positive regulator of nodulation. We propose that LjERF1 functions as a key regulator of successful infection of L. japonicus by Mesorhizobium loti.
|
巻・号 |
147(4)
|
ページ |
2030-40
|
公開日 |
2008-8-1
|
DOI |
10.1104/pp.108.118141
|
PII |
pp.108.118141
|
PMID |
18567832
|
PMC |
PMC2492631
|
MeSH |
Base Sequence
Cyclopentanes / pharmacology
Ethylenes / pharmacology
Gene Expression / drug effects
Gene Expression Profiling
Gene Expression Regulation, Plant / drug effects
Lotus / genetics*
Lotus / metabolism
Lotus / microbiology
Medicago truncatula / physiology
Molecular Sequence Data
Multigene Family
Oligonucleotide Array Sequence Analysis
Oxylipins / pharmacology
Peptide Termination Factors / analysis
Peptide Termination Factors / chemistry
Peptide Termination Factors / physiology*
Phylogeny
Plant Proteins / analysis
Plant Proteins / chemistry
Plant Proteins / physiology*
Protein Structure, Tertiary
RNA Interference
Root Nodules, Plant / genetics
Root Nodules, Plant / metabolism
Root Nodules, Plant / microbiology
Transcription Factors / analysis
Transcription Factors / chemistry
Transcription Factors / physiology
Up-Regulation
|
IF |
6.902
|
引用数 |
48
|
WOS 分野
|
PLANT SCIENCES
|
リソース情報 |
ミヤコグサ・ダイズ |
Gifu B-129 |