| RRC ID |
37499
|
| 著者 |
Mizowaki T, Sasayama T, Tanaka K, Mizukawa K, Takata K, Nakamizo S, Tanaka H, Nagashima H, Nishihara M, Hirose T, Itoh T, Kohmura E.
|
| タイトル |
STAT3 activation is associated with cerebrospinal fluid interleukin-10 (IL-10) in primary central nervous system diffuse large B cell lymphoma.
|
| ジャーナル |
J Neurooncol
|
| Abstract |
Signal transducers and activators of transcription 3 (STAT3) are activated by various cytokines and oncogenes; however, the activity and pathogenesis of STAT3 in diffuse large B cell lymphoma of the central nervous system have not been thoroughly elucidated. We investigated the phosphorylation levels of STAT3 in 40 specimens of primary central nervous system diffuse large B-cell lymphoma (PCNS DLBCL) and analyzed the association between phsopho-STAT3 (pSTAT3) expression and cerebrospinal fluid (CSF) concentration of interleukin-10 (IL-10) or IL-6. Immunohistochemistry and Western blot analysis revealed that most of the specimens in PCNS DLBCL expressed pSTST3 protein, and a strong phosphorylation levels of STAT3 was statistically associated with high CSF IL-10 levels, but not with CSF IL-6 levels. Next, we demonstrated that recombinant IL-10 and CSF containing IL-10 induced the phosphorylation of STAT3 in PCNS DLBCL cells. Furthermore, molecular subtype classified by Hans' algorithm was correlated with pSTAT3 expression levels and CSF IL-10 levels. These results suggest that the STAT3 activity is correlated with CSF IL-10 level, which is a useful marker for STAT3 activity in PCNS DLBCLs.
|
| 巻・号 |
124(2)
|
| ページ |
165-74
|
| 公開日 |
2015-9-1
|
| DOI |
10.1007/s11060-015-1843-9
|
| PII |
10.1007/s11060-015-1843-9
|
| PMID |
26080800
|
| MeSH |
Adult
Aged
Aged, 80 and over
Animals
Biomarkers / metabolism
Cell Line, Tumor
Central Nervous System Neoplasms / classification
Central Nervous System Neoplasms / diagnosis
Central Nervous System Neoplasms / metabolism*
Central Nervous System Neoplasms / pathology
Female
Humans
Interleukin-10 / metabolism*
Interleukin-6 / metabolism
Lymphoma, Large B-Cell, Diffuse / classification
Lymphoma, Large B-Cell, Diffuse / diagnosis
Lymphoma, Large B-Cell, Diffuse / metabolism*
Lymphoma, Large B-Cell, Diffuse / pathology
Male
Mice, Inbred BALB C
Mice, Nude
Middle Aged
Neoplasm Transplantation
Phosphorylation
Prognosis
Recombinant Proteins / metabolism
STAT3 Transcription Factor / metabolism*
|
| IF |
3.267
|
| 引用数 |
7
|
|
WOS 分野
|
CLINICAL NEUROLOGY
ONCOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
HKBML(RCB0820) |
