| RRC ID |
37605
|
| 著者 |
Kanda N, Anada T, Handa T, Kobayashi K, Ezoe Y, Takahashi T, Suzuki O.
|
| タイトル |
Orthotopic Osteogenecity Enhanced by a Porous Gelatin Sponge in a Critical-Sized Rat Calvaria Defect.
|
| ジャーナル |
Macromol Biosci
|
| Abstract |
The gelatin (Gel) powders, derived from acidic and basic extractions of porcine dermis (referred to as AE and BE), were processed for the porous sponge preparation. The disks, which were less than or greater than 500 μm in diameter [small (S) and large (L) pores, respectively] in both extractions and had an interconnected structure respectively, were implanted in critical-sized defects (CSD) of rat calvaria for 4 and 8 weeks to analyze the bone repair capability. Only the AE-S disk induced bone formation (over 60%) histomorphometrically in the CSD after 8 weeks, although the collagen orientation of the regenerated bone was still immature. Osteoblastic cell culture until 14 days did not substantiate marked superiority of AE-S disk regarding the proliferation and the differentiation, although the initial attachment was enhanced on AE-S disk than BE-L disk. The results provide the findings that a Gel sponge with a specific porous structure is capable of inducing orthotopic bone formation in vivo environment.
|
| 巻・号 |
15(12)
|
| ページ |
1647-55
|
| 公開日 |
2015-12-1
|
| DOI |
10.1002/mabi.201500191
|
| PMID |
26200698
|
| MeSH |
Animals
Bone Substitutes* / chemistry
Bone Substitutes* / pharmacology
Cell Differentiation / drug effects
Cell Proliferation / drug effects*
Cells, Cultured
Gelatin* / chemistry
Gelatin* / pharmacology
Male
Osteoblasts / metabolism*
Osteoblasts / pathology
Osteogenesis / drug effects*
Porosity
Rats
Rats, Wistar
Skull* / injuries
Skull* / metabolism
Skull* / pathology
Swine
|
| IF |
3.416
|
| 引用数 |
10
|
|
WOS 分野
|
POLYMER SCIENCE
BIOCHEMISTRY & MOLECULAR BIOLOGY
MATERIALS SCIENCE, BIOMATERIALS
|
| リソース情報 |
| ヒト・動物細胞 |
ST2(RCB0224) |
