論文 - 詳細
| RRC ID | 37672 |
|---|---|
| 著者 | Kawata K, Osawa M, Okabe S. |
| タイトル | In vitro toxicity of silver nanoparticles at noncytotoxic doses to HepG2 human hepatoma cells. |
| ジャーナル | Environ Sci Technol |
| Abstract |
Although it has been reported that silver nanoparticles (Ag-NPs) have strong acute toxic effects to various cultured cells, the toxic effects at noncytotoxic doses are still unknown. We, therefore, evaluated in vitro toxicity of Ag-NPs at noncytotoxic doses in human hepatoma cell line, HepG2, based on cell viability assay, micronucleus test, and DNA microarray analysis. We also used polystyrene nanoparticles (PS-NPs) and silver carbonate (Ag2CO3) as test materials to compare the toxic effects with respect to different raw chemical composition and form of silver. The cell viability assay demonstrated that Ag-NPs accelerated cell proliferation at low doses (< 0.5 mg/L), which was supported by the DNA microarray analysis showing significant induction of genes associated with cell cycle progression. However, only Ag-NPs exposure exhibited a significant cytotoxicity at higher doses (> 1.0 mg/L) and induced abnormal cellular morphology, displaying cellular shrinkage and acquisition of an irregular shape. In addition, only Ag-NPs exposure increased the frequency of micronucleus formation up to 47.9 +/- 3.2% of binucleated cells, suggesting that Ag-NPs appear to cause much stronger damages to chromosome than PS-NPs and ionic Ag+. Cysteine, a strong ionic Ag+ ligand, only partially abolished the formation of micronuclei mediated by Ag-NPs and changed the gene expression, indicating that ionic Ag+ derived from Ag-NPs could not fully explain these biological actions. Based on these discussions, it is concluded that both "nanosized particle of Ag" as well as "ionic Ag+" contribute to the toxic effects of Ag-NPs. |
| 巻・号 | 43(15) |
| ページ | 6046-51 |
| 公開日 | 2009-8-1 |
| DOI | 10.1021/es900754q |
| PMID | 19731716 |
| MeSH | Cell Line, Tumor Cysteine / chemistry DNA Damage Drug Screening Assays, Antitumor / methods* Gene Expression Profiling Gene Expression Regulation, Neoplastic / drug effects* Humans Ions Metal Nanoparticles / chemistry* Metal Nanoparticles / toxicity* Micronucleus Tests / methods Nanotechnology / methods Oligonucleotide Array Sequence Analysis Oxidative Stress Reactive Oxygen Species Silver / chemistry* Silver / toxicity* |
| IF | 7.864 |
| 引用数 | 452 |
| WOS 分野 | ENVIRONMENTAL SCIENCES ENGINEERING, ENVIRONMENTAL |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Hep G2 |