論文 - 詳細
| RRC ID | 37690 |
|---|---|
| 著者 | Mizukami S, Okada S, Kimura S, Kikuchi K. |
| タイトル | Design and synthesis of coumarin-based Zn(2+) probes for ratiometric fluorescence imaging. |
| ジャーナル | Inorg Chem |
| Abstract |
The physiological roles of free Zn(2+) have attracted great attention. To clarify those roles, there has been a need for ratiometric fluorescent Zn(2+) probes for practical use. We report the rational design and synthesis of a series of ratiometric fluorescent Zn(2+) probes. The structures of the probes are based on the 7-hydroxycoumarin structure. We focused on the relationship between the electron-donating ability of the 7-hydroxy group and the excitation spectra of 7-hydroxycoumarins, and exploited that relationship in the design of the ratiometric probes; as a result, most of the synthesized probes showed ratiometric Zn(2+)-sensing properties. Then, we designed and synthesized ratiometric Zn(2+) probes that can be excited with visible light, by choosing adequate substituents on coumarin dyes. Since one of the probes could permeate living cell membranes, we introduced the probe to living RAW264 cells and observed the intracellular Zn(2+) concentration via ratiometric fluorescence microscopy. As a result, the ratio value of the probe changed quickly in response to intracellular Zn(2+) concentration. |
| 巻・号 | 48(16) |
| ページ | 7630-8 |
| 公開日 | 2009-8-17 |
| DOI | 10.1021/ic900247r |
| PMID | 19591460 |
| MeSH | Animals Cell Line Cell Membrane Permeability Cell Survival Drug Design Fluorescent Dyes / chemical synthesis* Fluorescent Dyes / chemistry* Fluorescent Dyes / metabolism Hydrogen-Ion Concentration Spectrometry, Fluorescence Umbelliferones / chemical synthesis* Umbelliferones / chemistry* Umbelliferones / metabolism Zinc / analysis* Zinc / chemistry Zinc / metabolism |
| IF | 4.825 |
| 引用数 | 99 |
| WOS 分野 | CHEMISTRY, INORGANIC & NUCLEAR |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |