論文 - 詳細
| RRC ID | 377 |
|---|---|
| 著者 | Kawakami K, Takeda H, Kawakami N, Kobayashi M, Matsuda N, Mishina M. |
| タイトル | A transposon-mediated gene trap approach identifies developmentally regulated genes in zebrafish. |
| ジャーナル | Dev Cell |
| Abstract |
We report here development of a novel gene trap method in zebrafish using the Tol2 transposon system. First, we established a highly efficient transgenesis method in which a plasmid DNA containing the Tol2 transposon vector and the transposase mRNA synthesized in vitro were coinjected into one-cell stage embryos. The transposon vector inserted in the genome could be transmitted to the F1 progeny at high frequencies, and regulated gene expression by a specific promoter could be recapitulated in transgenic fish. Then we constructed a transposon-based gene trap vector containing a splice acceptor and the GFP gene, performed a pilot screen for gene trapping, and obtained fish expressing GFP in temporally and spatially restricted patterns. We confirmed the endogenous transcripts were indeed trapped by the insertions, and the insertion could interfere with expression of the trapped gene. We propose our gene trap approach should facilitate studies of vertebrate development and organogenesis. |
| 巻・号 | 7(1) |
| ページ | 133-44 |
| 公開日 | 2004-7-1 |
| DOI | 10.1016/j.devcel.2004.06.005 |
| PII | S1534-5807(04)00205-9 |
| PMID | 15239961 |
| MeSH | Animals Animals, Genetically Modified Base Sequence / genetics DNA Transposable Elements / genetics* Gene Expression Regulation, Developmental / genetics* Gene Transfer Techniques Genetic Vectors / genetics Genome Green Fluorescent Proteins Luminescent Proteins / genetics Molecular Sequence Data Plasmids / genetics RNA Splicing / genetics RNA, Messenger / pharmacology Transcription, Genetic / genetics Transgenes / genetics Transposases / genetics Zebrafish / embryology* Zebrafish / genetics* Zebrafish / metabolism Zebrafish Proteins / genetics* Zebrafish Proteins / metabolism* |
| IF | 10.092 |
| 引用数 | 554 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 13 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| ゼブラフィッシュ | SAG2A, SAG4A, SAG4B, SAG10A, SAG11A, SAG14A, SAG15A, SAG18A, SAG56A, SAG86A, SAG92A, SAGm11A, SAGm11B, SAGm14A, SAGm17A, SAGm17C, SAGm18A SAGm18B, SAGm18C, SAGp11A, SAGp17A, SAGp22B, SAGp47A, SAGp47B, SAGp49A, SAGp49B, SAGp53A, SAGp53B, SAGp57A, SAG20A, SAGp22A, SAGm17B, SAGp4A, SAGp33A, SAGp61A |