RRC ID 3770
著者 Yuksel B, Memon AR.
タイトル Comparative phylogenetic analysis of small GTP-binding genes of model legume plants and assessment of their roles in root nodules.
ジャーナル J Exp Bot
Abstract Small GTP-binding genes play an essential regulatory role in a multitude of cellular processes such as vesicle-mediated intracellular trafficking, signal transduction, cytoskeletal organization, and cell division in plants and animals. Medicago truncatula and Lotus japonicus are important model plants for studying legume-specific biological processes such as nodulation. The publicly available online resources for these plants from websites such as http://www.ncbi.nih.gov, http://www.medicago.org, http://www.tigr.org, and related sites were searched to collect nucleotide sequences that encode GTP-binding protein homologues. A total of 460 small GTPase sequences from several legume species including Medicago and Lotus, Arabidopsis, human, and yeast were phyletically analysed to shed light on the evolution and functional characteristics of legume-specific homologues. One of the main emphases of this study was the elucidation of the possible involvement of some members of small GTPase homologues in the establishment and maintenance of symbiotic associations in root nodules of legumes. A high frequency of vesicle-mediated trafficking in nodules led to the idea of a probable subfunctionalization of some members of this family in legumes. As a result of the analyses, a group of 10 small GTPases that are likely to be mainly expressed in nodules was determined. The sequences determined as a result of this study could be used in more detailed molecular genetic analyses such as creation of RNA interference silencing mutants for further clarification of the role of GTPases in nodulation. This study will also assist in furthering our understanding of the evolutionary history of small GTPases in legume species.
巻・号 59(14)
ページ 3831-44
公開日 2008-1-1
DOI 10.1093/jxb/ern223
PII ern223
PMID 18849296
PMC PMC2576638
MeSH Evolution, Molecular Fabaceae / classification* Fabaceae / enzymology* Fabaceae / genetics Gene Expression Regulation, Plant Humans Molecular Sequence Data Monomeric GTP-Binding Proteins / genetics* Monomeric GTP-Binding Proteins / metabolism Phylogeny* Plant Proteins / genetics* Plant Proteins / metabolism Root Nodules, Plant / classification* Root Nodules, Plant / enzymology* Root Nodules, Plant / genetics Sequence Homology Yeasts / classification Yeasts / genetics
IF 5.908
引用数 18
WOS 分野 PLANT SCIENCES
リソース情報
情報 Lotus