| RRC ID |
3773
|
| 著者 |
Shi Q, Parks AR, Potter BD, Safir IJ, Luo Y, Forster BM, Peters JE.
|
| タイトル |
DNA damage differentially activates regional chromosomal loci for Tn7 transposition in Escherichia coli.
|
| ジャーナル |
Genetics
|
| Abstract |
The bacterial transposon Tn7 recognizes replicating DNA as a target with a preference for the region where DNA replication terminates in the Escherichia coli chromosome. It was previously shown that DNA double-strand breaks in the chromosome stimulate Tn7 transposition where transposition events occur broadly around the point of the DNA break. We show that individual DNA breaks actually activate a series of small regional hotspots in the chromosome for Tn7 insertion. These hotspots are fixed and become active only when a DNA break occurs in the same region of the chromosome. We find that the distribution of insertions around the break is not explained by the exonuclease activity of RecBCD moving the position of the DNA break, and stimulation of Tn7 transposition is not dependent on RecBCD. We show that other forms of DNA damage, like exposure to UV light, mitomycin C, or phleomycin, also stimulate Tn7 transposition. However, inducing the SOS response does not stimulate transposition. Tn7 transposition is not dependent on any known specific pathway of replication fork reactivation as a means of recognizing DNA break repair. Our results are consistent with the idea that Tn7 recognizes DNA replication involved in DNA repair and reveals discrete regions of the chromosome that are differentially activated as transposition targets.
|
| 巻・号 |
179(3)
|
| ページ |
1237-50
|
| 公開日 |
2008-7-1
|
| DOI |
10.1534/genetics.108.088161
|
| PII |
genetics.108.088161
|
| PMID |
18562643
|
| PMC |
PMC2475729
|
| MeSH |
Chromosomes, Bacterial / genetics*
DNA Breaks, Double-Stranded / drug effects
DNA Breaks, Double-Stranded / radiation effects
DNA Damage*
DNA Helicases / metabolism
DNA Replication / drug effects
DNA Replication / radiation effects
DNA Transposable Elements / genetics*
Escherichia coli / drug effects
Escherichia coli / genetics*
Escherichia coli Proteins / metabolism
Mitomycin / pharmacology
Models, Genetic
Phleomycins / pharmacology
SOS Response, Genetics / drug effects
SOS Response, Genetics / radiation effects
Selection, Genetic
Ultraviolet Rays
|
| IF |
4.015
|
| 引用数 |
12
|
|
WOS 分野
|
GENETICS & HEREDITY
|
| リソース情報 |
| 情報 |
PEC |
