論文 - 詳細
| RRC ID | 37737 |
|---|---|
| 著者 | Tsunoda N, Kokuryo T, Oda K, Senga T, Yokoyama Y, Nagino M, Nimura Y, Hamaguchi M. |
| タイトル | Nek2 as a novel molecular target for the treatment of breast carcinoma. |
| ジャーナル | Cancer Sci |
| Abstract |
We investigated the role of Nek2, a member of the serine-threonine kinase family, in the tumorigenic growth of breast carcinoma. Increased expression of Nek2 was observed in all breast carcinoma cell lines examined (BT20, BT474, Hs578T, MCF7, MDA-MB-231, T47D, and ZR-75-1) by immunoblotting. By treatment with Nek2 short interfering RNA (siRNA), expression of Nek2 was clearly decreased in both estrogen receptor (ER)-positive (MCF7) and ER-negative (MDA-MB-231) breast carcinoma cell lines. Cell growth, colony formation in soft agar, and in vitro invasiveness of these cell lines were substantially suppressed by Nek2 siRNA treatment. In a xenograft nude mouse model with subcutaneous implantation of MCF7 or MDA-MB-231, subcutaneous injection of Nek2 siRNA around the tumor nodules resulted in a reduction of tumor size compared with those of control siRNA injection. Taken together, Nek2 appears to play a pivotal role in tumorigenic growth of breast carcinoma cells, and could be a useful therapeutic molecular target for the treatment of breast carcinoma both in ER-positive and ER-negative cases. |
| 巻・号 | 100(1) |
| ページ | 111-6 |
| 公開日 | 2009-1-1 |
| DOI | 10.1111/j.1349-7006.2008.01007.x |
| PII | CAS1007 |
| PMID | 19038001 |
| MeSH | Animals Breast Neoplasms / chemistry Breast Neoplasms / pathology Breast Neoplasms / therapy* Cell Line, Tumor Female Humans Male Mice NIMA-Related Kinases Protein Serine-Threonine Kinases / antagonists & inhibitors* Protein Serine-Threonine Kinases / genetics Protein Serine-Threonine Kinases / physiology RNA, Small Interfering / genetics Receptors, Estrogen / analysis |
| IF | 4.966 |
| 引用数 | 77 |
| WOS 分野 | ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | |