Reference - Detail
RRC ID | 37784 |
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Author | Tanaka M, Watanabe Y, Yoshimoto K. |
Title | Regulation of relaxin 3 gene expression via cAMP-PKA in a neuroblastoma cell line. |
Journal | J Neurosci Res |
Abstract |
Relaxin 3 is expressed in neurons of the brain stem that inneravate wide areas of the forebrain. Relaxin 3 mRNA levels in these neurons are increased in response to restraint stress, and by central administration of corticotropin-releasing factor (CRF). In the present study, we observed that relaxin 3 was expressed in a mouse neuroblastoma cell line, Neuro2a, and investigated the intracellular signaling that activated relaxin 3 gene transcription in vitro. By means of a clone stably transfected with a relaxin 3 promoter-EGFP gene, we observed that dibutyryl cyclic AMP and forskolin increased the relaxin 3 promoter activity. These increases were inhibited by pretreatment with PKA inhibitors, H89 and KT5720. Moreover, the promoter activity was enhanced by CRF treatment after expression of CRF-R1 receptor on the cells. Taken together, these results indicate that relaxin 3 transcription is activated via the cAMP-PKA pathway in the downstream of CRF-R1. |
Volume | 87(3) |
Pages | 820-9 |
Published | 2009-2-15 |
DOI | 10.1002/jnr.21895 |
PMID | 18831067 |
MeSH | Animals Base Sequence Brain Stem / metabolism Bucladesine / pharmacology* Carbazoles / pharmacology Cell Line, Tumor Colforsin / pharmacology* Conserved Sequence Corticotropin-Releasing Hormone / pharmacology Gene Expression Intracellular Signaling Peptides and Proteins / pharmacology* Isoquinolines / pharmacology Male Mice Mice, Inbred C57BL Molecular Sequence Data Promoter Regions, Genetic / drug effects* Pyrroles / pharmacology Receptors, Corticotropin-Releasing Hormone / metabolism Relaxin / genetics Relaxin / metabolism* Sulfonamides / pharmacology |
IF | 4.699 |
Times Cited | 11 |
WOS Category | NEUROSCIENCES |
Resource | |
Human and Animal Cells |