論文 - 詳細
| RRC ID | 37817 |
|---|---|
| 著者 | Horie I, Maeda M, Yokoyama S, Hisatsune A, Katsuki H, Miyata T, Isohama Y. |
| タイトル | Tumor necrosis factor-alpha decreases aquaporin-3 expression in DJM-1 keratinocytes. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Aquaporin-3 (AQP3) is a water/glycerol-transporting protein that is strongly expressed at the plasma membranes of keratinocytes in skin. There is evidence for involvement of AQP3-facilitated water and glycerol transport in skin hydration and wound repair, respectively. In this study, we show that tumor necrosis factor-alpha (TNF-alpha) and TNF receptor-1 signaling decreased AQP3 protein expression and plasma membrane water permeability in DJM-1 keratinocytes. TNF-alpha also decreased AQP3 mRNA expression and promoter activity, indicating that TNF-alpha suppresses AQP3 gene transcription. In addition, inhibitors of p38 and extracellular signal-regulated kinase (ERK) abolished the effect of TNF-alpha on AQP3 expression level, whereas inhibitors for NF-kappaB did not. These data indicate that TNF-alpha decreases AQP3 gene expression through p38 and ERK activation, and suggest that the decrease in AQP3 expression caused by TNF-alpha might be related to the phenotypes of skin inflammation, such as dry skin. |
| 巻・号 | 387(3) |
| ページ | 564-8 |
| 公開日 | 2009-9-25 |
| DOI | 10.1016/j.bbrc.2009.07.077 |
| PII | S0006-291X(09)01413-2 |
| PMID | 19619514 |
| MeSH | Aquaporin 3 / biosynthesis* Aquaporin 3 / genetics Cell Line Extracellular Signal-Regulated MAP Kinases / metabolism Gene Expression Regulation* Glycerol / metabolism Humans Keratinocytes / drug effects Keratinocytes / metabolism* Promoter Regions, Genetic RNA Stability RNA, Messenger / biosynthesis Transcription, Genetic Tumor Necrosis Factor-alpha / metabolism* Tumor Necrosis Factor-alpha / pharmacology Water / metabolism p38 Mitogen-Activated Protein Kinases / metabolism |
| IF | 2.985 |
| 引用数 | 24 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | DJM-1(RCB0736) |