論文 - 詳細
| RRC ID | 37820 |
|---|---|
| 著者 | Ushijima Y, Goshima F, Kimura H, Nishiyama Y. |
| タイトル | Herpes simplex virus type 2 tegument protein UL56 relocalizes ubiquitin ligase Nedd4 and has a role in transport and/or release of virions. |
| ジャーナル | Virol J |
| Abstract |
BACKGROUND:The ubiquitin system functions in a variety of cellular processes including protein turnover, protein sorting and trafficking. Many viruses exploit the cellular ubiquitin system to facilitate viral replication. In fact, herpes simplex virus (HSV) encodes a ubiquitin ligase (E3) and a de-ubiquitinating enzyme to modify the host's ubiquitin system. We have previously reported HSV type 2 (HSV-2) tegument protein UL56 as a putative adaptor protein of neuronal precursor cell-expressed developmentally down-regulated 4 (Nedd4) E3 ligase, which has been shown to be involved in protein sorting and trafficking. RESULTS:In this study, we visualized and characterized the dynamic intracellular localization of UL56 and Nedd4 using live-cell imaging and immunofluorescence analysis. UL56 was distributed to cytoplasmic vesicles, primarily to the trans-Golgi network (TGN), and trafficked actively throughout the cytoplasm. Moreover, UL56 relocalized Nedd4 to the vesicles in cells transiently expressing UL56 and in cells infected with HSV-2. We also investigated whether UL56 influenced the efficiency of viral replication, and found that extracellular infectious viruses were reduced in the absence of UL56. CONCLUSION:These data suggest that UL56 regulates Nedd4 and functions to facilitate the cytoplasmic transport of virions from TGN to the plasma membrane and/or release of virions from the cell surface. |
| 巻・号 | 6 |
| ページ | 168 |
| 公開日 | 2009-10-16 |
| DOI | 10.1186/1743-422X-6-168 |
| PII | 1743-422X-6-168 |
| PMID | 19835589 |
| PMC | PMC2770495 |
| MeSH | Animals Cell Membrane / chemistry Chlorocebus aethiops Cytoplasmic Vesicles / chemistry Endosomal Sorting Complexes Required for Transport / metabolism* Golgi Apparatus / chemistry Herpesvirus 2, Human / physiology* Microscopy, Confocal Microscopy, Fluorescence Models, Biological Nedd4 Ubiquitin Protein Ligases Protein Transport Ubiquitin-Protein Ligases / metabolism* Vero Cells Viral Proteins / metabolism* Virus Assembly* |
| IF | 2.579 |
| 引用数 | 18 |
| WOS 分野 | VIROLOGY |
| リソース情報 | |
| ヒト・動物細胞 | Vero(RCB0001) |