| RRC ID |
37848
|
| 著者 |
Ishikawa A, Kato T, Susa T, Sano A, Kato Y.
|
| タイトル |
Molecular cloning and characterization of porcine homeodomain transcription factor Msx1.
|
| ジャーナル |
J Reprod Dev
|
| Abstract |
We cloned a porcine ortholog of homeodomain transcription factor Msx1 from the porcine pituitary cDNA library. The amino acid sequence of Msx1 shows high conservation among mammalian species. RT-PCR for porcine fetal and postnatal pituitaries showed that Msx1 is already expressed at early fetal day 40, decreases to a low level before birth and then remarkably decreases after birth. On the other hand, Msx1 expression was observed in all pituitary-derived cell line tested, with most in a gonadotrope lineage LbetaT4. Transfection assay demonstrated that Msx1 markedly repressed the basal Cga and Fshb gene expression, while Lhb expression was affected slightly. Taken together, Msx1 may play a role in repressing gene expression in the fetal and postnatal periods.
|
| 巻・号 |
55(3)
|
| ページ |
278-82
|
| 公開日 |
2009-6-1
|
| DOI |
10.1262/jrd.20179
|
| PII |
JST.JSTAGE/jrd/20179
|
| PMID |
19262020
|
| MeSH |
Amino Acid Sequence
Animals
Cells, Cultured
Cloning, Molecular*
Embryo, Mammalian
Female
Gene Expression Regulation, Developmental
Gene Silencing
Homeodomain Proteins / genetics
Homeodomain Proteins / isolation & purification
MSX1 Transcription Factor / genetics*
MSX1 Transcription Factor / isolation & purification
MSX1 Transcription Factor / metabolism
Male
Mice
Molecular Sequence Data
Pituitary Gland / embryology
Pituitary Gland / metabolism
Sequence Homology, Amino Acid
Swine / embryology
Swine / genetics*
|
| IF |
1.735
|
| 引用数 |
5
|
|
WOS 分野
|
AGRICULTURE, DAIRY & ANIMAL SCIENCE
REPRODUCTIVE BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
CHO-K1(RCB0285) |
