| RRC ID |
37857
|
| 著者 |
Yamasaki M, Kawabe A, Nishimoto K, Madhyastha H, Sakakibara Y, Suiko M, Okamoto T, Suda T, Uehira K, Nishiyama K.
|
| タイトル |
Dihydro-alpha-lipoic acid has more potent cytotoxicity than alpha-lipoic acid.
|
| ジャーナル |
In Vitro Cell Dev Biol Anim
|
| Abstract |
Alpha-lipoic acid has been shown to possess cancer-cell-killing activity via activation of the apoptosis pathway. In this study, the cytotoxic activities of alpha-lipoic and dihydro-alpha-lipoic acid were compared in HL-60 cells. The cell-killing activity of dihydro-alpha-lipoic acid was higher than that of alpha-lipoic acid. Both alpha-lipoic and dihydro-alpha-lipoic acid induced caspase-3 cleavage and internucleosomal DNA fragmentation in treated cells. On the other hand, apparent necrotic or late-stage apoptotic cell populations could be detected in dihydro-alpha-lipoic acid cells but not in those treated with alpha-lipoic acid. Moreover, dihydro-alpha-lipoic acid, but not alpha-lipoic acid, induced marked mitochondrial permeability transition. Antioxidants could not prevent dihydro-alpha-lipoic- or alpha-lipoic-acid-induced cell death. In addition, dihydro-alpha-lipoic and alpha-lipoic acid did not up-regulate cellular reactive oxygen level. These results indicated that dihydro-alpha-lipoic acid exerts more potent cytotoxicity than alpha-lipoic acid through different cytotoxic actions.
|
| 巻・号 |
45(5-6)
|
| ページ |
275-80
|
| 公開日 |
2009-1-1
|
| DOI |
10.1007/s11626-008-9164-3
|
| PMID |
19118438
|
| MeSH |
Annexin A5 / metabolism
Caspase 3 / metabolism
Cell Death / drug effects
DNA Fragmentation / drug effects
Flow Cytometry
HL-60 Cells
Humans
Intracellular Space / drug effects
Intracellular Space / metabolism
Mitochondria / drug effects
Mitochondria / enzymology
Oxidation-Reduction / drug effects
Permeability / drug effects
Propidium / metabolism
Thioctic Acid / analogs & derivatives*
Thioctic Acid / pharmacology*
|
| IF |
1.665
|
| 引用数 |
15
|
|
WOS 分野
|
DEVELOPMENTAL BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
HL60(RCB0041) |
