論文 - 詳細
| RRC ID | 37857 |
|---|---|
| 著者 | Yamasaki M, Kawabe A, Nishimoto K, Madhyastha H, Sakakibara Y, Suiko M, Okamoto T, Suda T, Uehira K, Nishiyama K. |
| タイトル | Dihydro-alpha-lipoic acid has more potent cytotoxicity than alpha-lipoic acid. |
| ジャーナル | In Vitro Cell Dev Biol Anim |
| Abstract |
Alpha-lipoic acid has been shown to possess cancer-cell-killing activity via activation of the apoptosis pathway. In this study, the cytotoxic activities of alpha-lipoic and dihydro-alpha-lipoic acid were compared in HL-60 cells. The cell-killing activity of dihydro-alpha-lipoic acid was higher than that of alpha-lipoic acid. Both alpha-lipoic and dihydro-alpha-lipoic acid induced caspase-3 cleavage and internucleosomal DNA fragmentation in treated cells. On the other hand, apparent necrotic or late-stage apoptotic cell populations could be detected in dihydro-alpha-lipoic acid cells but not in those treated with alpha-lipoic acid. Moreover, dihydro-alpha-lipoic acid, but not alpha-lipoic acid, induced marked mitochondrial permeability transition. Antioxidants could not prevent dihydro-alpha-lipoic- or alpha-lipoic-acid-induced cell death. In addition, dihydro-alpha-lipoic and alpha-lipoic acid did not up-regulate cellular reactive oxygen level. These results indicated that dihydro-alpha-lipoic acid exerts more potent cytotoxicity than alpha-lipoic acid through different cytotoxic actions. |
| 巻・号 | 45(5-6) |
| ページ | 275-80 |
| 公開日 | 2009-1-1 |
| DOI | 10.1007/s11626-008-9164-3 |
| PMID | 19118438 |
| MeSH | Annexin A5 / metabolism Caspase 3 / metabolism Cell Death / drug effects DNA Fragmentation / drug effects Flow Cytometry HL-60 Cells Humans Intracellular Space / drug effects Intracellular Space / metabolism Mitochondria / drug effects Mitochondria / enzymology Oxidation-Reduction / drug effects Permeability / drug effects Propidium / metabolism Thioctic Acid / analogs & derivatives* Thioctic Acid / pharmacology* |
| IF | 1.665 |
| 引用数 | 15 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | HL60(RCB0041) |