論文 - 詳細
| RRC ID | 37882 |
|---|---|
| 著者 | Matsuura K, Seta H, Haraguchi Y, Alsayegh K, Sekine H, Shimizu T, Hagiwara N, Yamazaki K, Okano T. |
| タイトル | TRPV-1-mediated elimination of residual iPS cells in bioengineered cardiac cell sheet tissues. |
| ジャーナル | Sci Rep |
| Abstract |
The development of a suitable strategy for eliminating remaining undifferentiated cells is indispensable for the use of human-induced pluripotent stem (iPS) cell-derived cells in regenerative medicine. Here, we show for the first time that TRPV-1 activation through transient culture at 42 °C in combination with agonists is a simple and useful strategy to eliminate iPS cells from bioengineered cardiac cell sheet tissues. When human iPS cells were cultured at 42 °C, almost all cells disappeared by 48 hours through apoptosis. However, iPS cell-derived cardiomyocytes and fibroblasts maintained transcriptional and protein expression levels, and cardiac cell sheets were fabricated after reducing the temperature. TRPV-1 expression in iPS cells was upregulated at 42 °C, and iPS cell death at 42 °C was TRPV-1-dependent. Furthermore, TRPV-1 activation through thermal or agonist treatment eliminated iPS cells in cardiac tissues for a final concentration of 0.4% iPS cell contamination. These findings suggest that the difference in tolerance to TRPV-1 activation between iPS cells and iPS cell-derived cardiac cells could be exploited to eliminate remaining iPS cells in bioengineered cell sheet tissues, which will further reduce the risk of tumour formation. |
| 巻・号 | 6 |
| ページ | 21747 |
| 公開日 | 2016-2-18 |
| DOI | 10.1038/srep21747 |
| PII | srep21747 |
| PMID | 26888607 |
| PMC | PMC4757885 |
| MeSH | Cell Differentiation Cells, Cultured Coculture Techniques Fibroblasts / cytology Fibroblasts / metabolism Hot Temperature Humans Induced Pluripotent Stem Cells / cytology* Myocytes, Cardiac / cytology* Myocytes, Cardiac / metabolism TRPV Cation Channels / agonists TRPV Cation Channels / genetics* TRPV Cation Channels / metabolism* Tissue Engineering / methods* |
| IF | 3.998 |
| 引用数 | 14 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 22 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 201B7(HPS0063) 253G1(HPS0002) |