Reference - Detail
| RRC ID | 37947 |
|---|---|
| Author | Zhu Q, Bang TH, Ohnuki K, Sawai T, Sawai K, Shimizu K. |
| Title | Inhibition of neuraminidase by Ganoderma triterpenoids and implications for neuraminidase inhibitor design. |
| Journal | Sci Rep |
| Abstract |
Neuraminidase (NA) inhibitors are the dominant antiviral drugs for treating influenza in the clinic. Increasing prevalence of drug resistance makes the discovery of new NA inhibitors a high priority. Thirty-one triterpenoids from the medicinal mushroom Ganoderma lingzhi were analyzed in an in vitro NA inhibition assay, leading to the discovery of ganoderic acid T-Q and TR as two inhibitors of H5N1 and H1N1 NAs. Structure-activity relationship studies revealed that the corresponding triterpenoid structure is a potential scaffold for the design of NA inhibitors. Using these triterpenoids as probes we found, through further in silico docking and interaction analysis, that interactions with the amino-acid residues Arg292 and/or Glu119 of NA are critical for the inhibition of H5N1 and H1N1. These findings should prove valuable for the design and development of NA inhibitors. |
| Volume | 5 |
| Pages | 13194 |
| Published | 2015-8-26 |
| DOI | 10.1038/srep13194 |
| PII | srep13194 |
| PMID | 26307417 |
| PMC | PMC4549708 |
| MeSH | Binding Sites Drug Design* Enzyme Activation Ganoderma / metabolism* Models, Chemical Molecular Docking Simulation* Neuraminidase / antagonists & inhibitors* Neuraminidase / chemistry* Protein Binding Protein Conformation Structure-Activity Relationship Triterpenes / administration & dosage Triterpenes / chemistry |
| IF | 3.998 |
| Times Cited | 23 |
| WOS Category | PHARMACOLOGY & PHARMACY |
| Resource | |
| Human and Animal Cells | MCF7(RCB1904) |