論文 - 詳細
| RRC ID | 37949 |
|---|---|
| 著者 | Ishii T, Sato K, Kakumoto T, Miura S, Touhara K, Takeuchi S, Nakata T. |
| タイトル | Light generation of intracellular Ca(2+) signals by a genetically encoded protein BACCS. |
| ジャーナル | Nat Commun |
| Abstract |
Ca(2+) signals are highly regulated in a spatiotemporal manner in numerous cellular physiological events. Here we report a genetically engineered blue light-activated Ca(2+) channel switch (BACCS), as an optogenetic tool for generating Ca(2+) signals. BACCS opens Ca(2+)-selective ORAI ion channels in response to light. A BACCS variant, dmBACCS2, combined with Drosophila Orai, elevates the Ca(2+) concentration more rapidly, such that Ca(2+) elevation in mammalian cells is observed within 1 s on light exposure. Using BACCSs, we successfully control cellular events including NFAT-mediated gene expression. In the mouse olfactory system, BACCS mediates light-dependent electrophysiological responses. Furthermore, we generate BACCS mutants, which exhibit fast and slow recovery of intracellular Ca(2+). Thus, BACCSs are a useful optogenetic tool for generating temporally various intracellular Ca(2+) signals with a large dynamic range, and will be applicable to both in vitro and in vivo studies. |
| 巻・号 | 6 |
| ページ | 8021 |
| 公開日 | 2015-8-18 |
| DOI | 10.1038/ncomms9021 |
| PII | ncomms9021 |
| PMID | 26282514 |
| PMC | PMC4557345 |
| MeSH | Animals COS Cells Calcium / metabolism Calcium Channels / genetics Calcium Channels / metabolism* Calcium Signaling / physiology* Chlorocebus aethiops Electrophysiological Phenomena Genes, Transgenic, Suicide Genetic Engineering / methods* HEK293 Cells Humans Light* Luminescent Proteins Mice Olfactory Mucosa / metabolism |
| IF | 12.121 |
| 引用数 | 33 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 9 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 293(RCB1637) Jurkat(RCB0806) S2 (Drosophila)(RCB1153) |