RRC ID 37949
著者 Ishii T, Sato K, Kakumoto T, Miura S, Touhara K, Takeuchi S, Nakata T.
タイトル Light generation of intracellular Ca(2+) signals by a genetically encoded protein BACCS.
ジャーナル Nat Commun
Abstract Ca(2+) signals are highly regulated in a spatiotemporal manner in numerous cellular physiological events. Here we report a genetically engineered blue light-activated Ca(2+) channel switch (BACCS), as an optogenetic tool for generating Ca(2+) signals. BACCS opens Ca(2+)-selective ORAI ion channels in response to light. A BACCS variant, dmBACCS2, combined with Drosophila Orai, elevates the Ca(2+) concentration more rapidly, such that Ca(2+) elevation in mammalian cells is observed within 1 s on light exposure. Using BACCSs, we successfully control cellular events including NFAT-mediated gene expression. In the mouse olfactory system, BACCS mediates light-dependent electrophysiological responses. Furthermore, we generate BACCS mutants, which exhibit fast and slow recovery of intracellular Ca(2+). Thus, BACCSs are a useful optogenetic tool for generating temporally various intracellular Ca(2+) signals with a large dynamic range, and will be applicable to both in vitro and in vivo studies.
巻・号 6
ページ 8021
公開日 2015-8-18
DOI 10.1038/ncomms9021
PII ncomms9021
PMID 26282514
PMC PMC4557345
MeSH Animals COS Cells Calcium / metabolism Calcium Channels / genetics Calcium Channels / metabolism* Calcium Signaling / physiology* Chlorocebus aethiops Electrophysiological Phenomena Genes, Transgenic, Suicide Genetic Engineering / methods* HEK293 Cells Humans Light* Luminescent Proteins Mice Olfactory Mucosa / metabolism
IF 12.121
引用数 33
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 293(RCB1637) Jurkat(RCB0806) S2 (Drosophila)(RCB1153)