| RRC ID |
379
|
| Author |
Hanaoka R, Ohmori Y, Uyemura K, Hosoya T, Hotta Y, Shirao T, Okamoto H.
|
| Title |
Zebrafish gcmb is required for pharyngeal cartilage formation.
|
| Journal |
Mech Dev
|
| Abstract |
The glial cells missing (gcm) gene in Drosophila encodes a GCM-motif transcription factor that functions as a binary switch to select between glial and neuronal cell fates. To understand the function of gcm in vertebrates, we isolated the zebrafish gcmb and analyzed the function of this gene using antisense morpholino oligonucleotides against gcmb mRNA (gcmb-MO) and transgenic overexpression. Zebrafish gcmb is expressed in the pharyngeal arch epithelium and in cells of the macrophage lineage. gcmb-MO-injected larvae show significantly reduced branchial arch cartilages. fgf3-MO-injected larvae display a similar phenotype to that of gcmb-MO-injected larvae with respect to the lack of pharyngeal cartilage formation. In addition, gcmb expression in the pharyngeal arches is down-regulated in fgf3-MO-injected larvae. The gcmb transgenic larvae show a protrusion of the lower jaw and abnormal spatial arrangement of the pharyngeal cartilage elements. These results suggest that gcmb is required for normal pharyngeal cartilage formation in zebrafish and that its expression is dependent on fgf3 activity.
|
| Volume |
121(10)
|
| Pages |
1235-47
|
| Published |
2004-10-1
|
| DOI |
10.1016/j.mod.2004.05.011
|
| PII |
S0925477304001376
|
| PMID |
15327784
|
| MeSH |
Amino Acid Sequence
Animals
Animals, Genetically Modified
Base Sequence
Branchial Region / growth & development*
Cartilage / growth & development*
Cloning, Molecular
DNA-Binding Proteins
Fibroblast Growth Factor 3
Fibroblast Growth Factors / genetics
Gene Expression Regulation, Developmental
In Situ Hybridization
Larva / drug effects
Larva / growth & development
Models, Biological
Molecular Sequence Data
Neuropeptides / genetics*
Oligodeoxyribonucleotides, Antisense / genetics
Oligodeoxyribonucleotides, Antisense / pharmacology
Phenotype
Sequence Homology, Amino Acid
Trans-Activators / genetics*
Transcription Factors
Zebrafish / genetics*
Zebrafish / growth & development*
Zebrafish Proteins / genetics*
|
| IF |
2.126
|
| Times Cited |
22
|
|
WOS Category
|
DEVELOPMENTAL BIOLOGY
|
| Resource |
| Zebrafish |
rw0128 |
