RRC ID 381
著者 Okamoto H, Hirate Y, Ando H.
タイトル Systematic identification of factors in zebrafish regulating the early midbrain and cerebellar development by odered differential display and caged mRNA technology.
ジャーナル Front Biosci
Abstract During brain development, various transcription factors are activated in the regional specific manner and define the identities characteristic to individual regions, and many of such factors have been identified in the vertebrate brain, by taking advantage of the structural and functional conservation of them with the invertebrate counterparts. However, it is still largely unknown why individual transcription factors can define the final morphology and function of the tissues expressing these factors because of the lack of knowledge on which genes are actually up- or down-regulated as downstream targets of individual transcription factors. In this review, we introduce novel technologies which we have invented or improved as a part of our endeavor to identify and functionally analyze the downstream target genes of Isle-3 which are involved in development of the midbrain and the midbrain/hindbrain boundary region in zebrafish embryos. Our strategy and technologies can be applied to analyzing the downstream genes of any other transcription factors.
巻・号 9
ページ 93-9
公開日 2004-1-1
DOI 10.2741/1205
PMID 14766348
MeSH Animals Biotechnology / methods* Cerebellum / embryology Cerebellum / physiology* Eye Proteins / genetics Eye Proteins / physiology* Homeodomain Proteins / genetics Homeodomain Proteins / physiology* LIM-Homeodomain Proteins Mesencephalon / embryology Mesencephalon / physiology* Nerve Tissue Proteins / genetics Nerve Tissue Proteins / physiology* RNA, Messenger / analysis Transcription Factors Zebrafish Zebrafish Proteins / genetics Zebrafish Proteins / physiology*
IF 2.747
引用数 5
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
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