論文 - 詳細
| RRC ID | 38107 |
|---|---|
| 著者 | Tateno H, Onuma Y, Ito Y, Minoshima F, Saito S, Shimizu M, Aiki Y, Asashima M, Hirabayashi J. |
| タイトル | Elimination of tumorigenic human pluripotent stem cells by a recombinant lectin-toxin fusion protein. |
| ジャーナル | Stem Cell Reports |
| Abstract |
The application of stem-cell-based therapies in regenerative medicine is hindered by the tumorigenic potential of residual human pluripotent stem cells. Previously, we identified a human pluripotent stem-cell-specific lectin probe, called rBC2LCN, by comprehensive glycome analysis using high-density lectin microarrays. Here we developed a recombinant lectin-toxin fusion protein of rBC2LCN with a catalytic domain of Pseudomonas aeruginosa exotoxin A, termed rBC2LCN-PE23, which could be expressed as a soluble form from the cytoplasm of Escherichia coli and purified to homogeneity by one-step affinity chromatography. rBC2LCN-PE23 bound to human pluripotent stem cells, followed by its internalization, allowing intracellular delivery of a cargo of cytotoxic protein. The addition of rBC2LCN-PE23 to the culture medium was sufficient to completely eliminate human pluripotent stem cells. Thus, rBC2LCN-PE23 has the potential to contribute to the safety of stem-cell-based therapies. |
| 巻・号 | 4(5) |
| ページ | 811-20 |
| 公開日 | 2015-5-12 |
| DOI | 10.1016/j.stemcr.2015.02.016 |
| PII | S2213-6711(15)00072-7 |
| PMID | 25866158 |
| PMC | PMC4437484 |
| MeSH | ADP Ribose Transferases / genetics ADP Ribose Transferases / metabolism* Bacterial Toxins / genetics Bacterial Toxins / metabolism* Cell Differentiation / drug effects Cell Survival / drug effects Cell Transformation, Neoplastic / drug effects Escherichia coli / metabolism Exotoxins / genetics Exotoxins / metabolism* Humans Lectins / genetics Lectins / metabolism* Plasmids / metabolism Pluripotent Stem Cells / cytology Pluripotent Stem Cells / drug effects Pluripotent Stem Cells / metabolism* Recombinant Fusion Proteins / biosynthesis Recombinant Fusion Proteins / isolation & purification Recombinant Fusion Proteins / pharmacology Virulence Factors / genetics Virulence Factors / metabolism* |
| IF | 6.032 |
| 引用数 | 54 |
| WOS 分野 | CELL & TISSUE ENGINEERING CELL BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | 253G1(HPS0002) 201B7(HPS0063) |