Reference - Detail
| RRC ID | 38134 |
|---|---|
| Author | Yamada M, Hori A, Sugaya S, Yajima Y, Utoh R, Yamato M, Seki M. |
| Title | Cell-sized condensed collagen microparticles for preparing microengineered composite spheroids of primary hepatocytes. |
| Journal | Lab Chip |
| Abstract |
The reconstitution of extracellular matrix (ECM) components in three-dimensional (3D) cell culture environments with microscale precision is a challenging issue. ECM microparticles would potentially be useful as solid particulate scaffolds that can be incorporated into 3D cellular constructs, but technologies for transforming ECM proteins into cell-sized stable particles are currently lacking. Here, we describe new processes to produce highly condensed collagen microparticles by means of droplet microfluidics or membrane emulsification. Droplets of an aqueous solution of type I collagen were formed in a continuous phase of polar organic solvent followed by rapid dissolution of water molecules into the continuous phase because the droplets were in a non-equilibrium state. We obtained highly unique, disc-shaped condensed collagen microparticles with a final collagen concentration above 10% and examined factors affecting particle size and morphology. After testing the cell-adhesion properties on the collagen microparticles, composite multicellular spheroids comprising the particles and primary rat hepatocytes were formed using microfabricated hydrogel chambers. We found that the ratio of the cells and particles is critical in terms of improvement of hepatic functions in the composite spheroids. The presented methodology for incorporating particulate-form ECM components in multicellular spheroids would be advantageous because of the biochemical similarity with the microenvironments in vivo. |
| Volume | 15(19) |
| Pages | 3941-51 |
| Published | 2015-10-7 |
| DOI | 10.1039/c5lc00785b |
| PMID | 26308935 |
| MeSH | Animals Cell Adhesion Cells, Cultured Collagen Type I / chemistry* Cytochrome P-450 CYP3A / genetics Cytochrome P-450 CYP3A / metabolism Hep G2 Cells Hepatocytes / cytology* Hepatocytes / metabolism Humans Hydrogel, Polyethylene Glycol Dimethacrylate / chemistry Mice Microfluidic Analytical Techniques / instrumentation Microfluidic Analytical Techniques / methods Microscopy, Fluorescence Microspheres* NIH 3T3 Cells Ornithine Carbamoyltransferase / genetics Ornithine Carbamoyltransferase / metabolism Rats Real-Time Polymerase Chain Reaction Spheroids, Cellular / cytology* Spheroids, Cellular / metabolism |
| IF | 6.774 |
| Times Cited | 37 |
| WOS Category | BIOCHEMICAL RESEARCH METHODS CHEMISTRY, MULTIDISCIPLINARY NANOSCIENCE & NANOTECHNOLOGY |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 7 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | NIH3T3 Hep G2 |