論文 - 詳細
| RRC ID | 38213 |
|---|---|
| 著者 | Fujii R, Okabe S, Urushido T, Inoue K, Yoshimura A, Tachibana T, Nishikawa T, Hicks GG, Takumi T. |
| タイトル | The RNA binding protein TLS is translocated to dendritic spines by mGluR5 activation and regulates spine morphology. |
| ジャーナル | Curr Biol |
| Abstract |
Neuronal dendrites, together with dendritic spines, exhibit enormously diverse structure. Selective targeting and local translation of mRNAs in dendritic spines have been implicated in synapse remodeling or synaptic plasticity. The mechanism of mRNA transport to the postsynaptic site is a fundamental question in local dendritic translation. TLS (translocated in liposarcoma), previously identified as a component of hnRNP complexes, unexpectedly showed somatodendritic localization in mature hippocampal pyramidal neurons. In the present study, TLS was translocated to dendrites and was recruited to dendrites not only via microtubules but also via actin filaments. In mature hippocampal pyramidal neurons, TLS accumulated in the spines at excitatory postsynapses upon mGluR5 activation, which was accompanied by an increased RNA content in dendrites. Consistent with the in vitro studies, TLS-null hippocampal pyramidal neurons exhibited abnormal spine morphology and lower spine density. Our results indicate that TLS participates in mRNA sorting to the dendritic spines induced by mGluR5 activation and regulates spine morphology to stabilize the synaptic structure. |
| 巻・号 | 15(6) |
| ページ | 587-93 |
| 公開日 | 2005-3-29 |
| DOI | 10.1016/j.cub.2005.01.058 |
| PII | S0960-9822(05)00225-3 |
| PMID | 15797031 |
| MeSH | Actins / metabolism Animals Cells, Cultured Dendritic Spines / metabolism* Fluorescence Recovery After Photobleaching Green Fluorescent Proteins Hippocampus / metabolism* Immunohistochemistry Mice Microscopy, Confocal Microtubules / metabolism Neurons / cytology* Neurons / metabolism Protein Transport / physiology RNA, Messenger / metabolism* RNA-Binding Protein FUS / metabolism* Receptors, Kainic Acid / metabolism* |
| IF | 9.601 |
| 引用数 | 209 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | News |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pEGFP-hTLS (RDB14189) |