論文 - 詳細
| RRC ID | 38217 |
|---|---|
| 著者 | Tahara K, Yamamoto H, Hirashima N, Kawashima Y. |
| タイトル | Chitosan-modified poly(D,L-lactide-co-glycolide) nanospheres for improving siRNA delivery and gene-silencing effects. |
| ジャーナル | Eur J Pharm Biopharm |
| Abstract |
Chitosan (CS) surface-modified poly(D,L-lactide-co-glycolide) (PLGA) nanospheres (NS) for a siRNA delivery system were evaluated in vitro. siRNA-loaded PLGA NS were prepared by an emulsion solvent diffusion (ESD) method, and the physicochemical properties of NS were investigated. The level of targeted protein expression and siRNA uptake were examined in A549 cells. CS-modified PLGA NS exhibited much higher encapsulation efficiency than unmodified PLGA NS (plain-PLGA NS). CS-modified PLGA NS showed a positive zeta potential, while plain-PLGA NS were negatively charged. siRNA uptake studies by observation with confocal laser scanning microscopy (CLSM) indicated that siRNA-loaded CS-modified PLGA NS were more effectively taken up by the cells than plain-PLGA NS. The efficiencies of different siRNA preparations were compared at the level of targeted protein expression. The gene-silencing efficiency of CS-modified PLGA NS was higher and more prolonged than those of plain-PLGA NS and naked siRNA. This result correlated with the CLSM studies, which may have been due to higher cellular uptake of CS-modified PLGA NS due to electrostatic interactions. It was concluded that CS-modified PLGA NS containing siRNA could provide an effective siRNA delivery system. |
| 巻・号 | 74(3) |
| ページ | 421-6 |
| 公開日 | 2010-3-1 |
| DOI | 10.1016/j.ejpb.2009.12.007 |
| PII | S0939-6411(09)00360-9 |
| PMID | 20034563 |
| PMC | PMC7127408 |
| MeSH | Cell Culture Techniques Cell Line, Tumor Chitosan / chemistry* Drug Carriers / chemistry* Drug Compounding Gene Silencing / drug effects* Humans Lactic Acid / chemistry* Luciferases, Firefly / genetics Microscopy, Confocal Microscopy, Electron, Scanning Nanospheres / chemistry* Particle Size Plasmids Polyglycolic Acid / chemistry* Polylactic Acid-Polyglycolic Acid Copolymer RNA, Small Interfering / administration & dosage* RNA, Small Interfering / pharmacology Surface Properties Transfection |
| IF | 4.604 |
| 引用数 | 56 |
| WOS 分野 | PHARMACOLOGY & PHARMACY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | A549(RCB0098) |