RRC ID |
3825
|
著者 |
Nakamura S, Aoki Y, Saito D, Kuroki Y, Fujiyama A, Naruse K, Tanaka M.
|
タイトル |
Sox9b/sox9a2-EGFP transgenic medaka reveals the morphological reorganization of the gonads and a common precursor of both the female and male supporting cells.
|
ジャーナル |
Mol Reprod Dev
|
Abstract |
We have established an enhanced green fluorescent protein (EGFP) transgenic medaka line that mimics the expression of sox9b/sox9a2 to analyze the morphological reorganization of the gonads and characterize the sox9b-expressing cells during gonadal formation in this fish. After the germ cells have migrated into the gonadal areas, a cluster of EGFP-expressing cells in the single gonadal primordium was found to be separated by the somatic cells along the rostrocaudal axis and form the bilateral lobes. We observed in these transgenic fish that EGFP expression persists only in the somatic cells directly surrounding the germ cells. As sex differentiation proceeds, dmrt1 and foxl2 begin to be expressed in the EGFP-expressing cells in the XY and the XX gonads, respectively. This indicates that the sox9b-expressing cells reorganize into two lobes of the gonad and then differentiate into Sertoli or granulosa cells, as common precursors of the supporting cells. Hence, our sox9b-EGFP medaka system will be useful in future studies of gonadal development.
|
巻・号 |
75(3)
|
ページ |
472-6
|
公開日 |
2008-3-1
|
DOI |
10.1002/mrd.20764
|
PMID |
17474097
|
MeSH |
Animals
Animals, Genetically Modified
Embryo, Nonmammalian
Female
Gene Expression Regulation, Developmental
Gonads / embryology*
Gonads / metabolism
Granulosa Cells / cytology*
Granulosa Cells / metabolism
Granulosa Cells / physiology
Green Fluorescent Proteins / genetics*
Green Fluorescent Proteins / metabolism
High Mobility Group Proteins / genetics*
High Mobility Group Proteins / metabolism
Male
Oryzias / embryology
Oryzias / genetics*
Protein Isoforms / genetics
Protein Isoforms / metabolism
SOX9 Transcription Factor
Sertoli Cells / cytology*
Sertoli Cells / metabolism
Sertoli Cells / physiology
Transcription Factors / genetics*
Transcription Factors / metabolism
|
IF |
2.823
|
引用数 |
55
|
WOS 分野
|
REPRODUCTIVE BIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
DEVELOPMENTAL BIOLOGY
CELL BIOLOGY
|
リソース情報 |
メダカ |
BAC (ola1-014G01)
inbred cab strain |