RRC ID 38267
Author Makihira S, Mine Y, Nikawa H, Shuto T, Iwata S, Hosokawa R, Kamoi K, Okazaki S, Yamaguchi Y.
Title Titanium ion induces necrosis and sensitivity to lipopolysaccharide in gingival epithelial-like cells.
Journal Toxicol In Vitro
Abstract Gingival epithelial-like cells (GE-1) were cultured and used to examine the cellular responses of gingival tissues to varying concentrations of titanium (Ti) ions. Titanium ions at concentrations of more than 13 ppm significantly decreased the viability of GE-1 cells and increased LDH release from the cells into the supernatant, but had no significant effect on their caspase 3 activity. These data suggest that a high concentration of Ti ions induced necrosis of the GE-1 cells. Titanium ions at a concentration of 5 ppm significantly increased the level of CCL2 mRNA expression in GE-1 cells exposed to lipopolysaccharide derived from Porphyromonas gingivalis in a synergistic manner. Moreover, the mRNA expression levels of TLR-4 and ICAM-1 in GE-1 cells loaded with Ti ions at 9 ppm were significantly enhanced as compared with those in GE-1 cells without Ti stimulation. We suggest that Ti ions are in part responsible for monocyte infiltration in the oral cavity by elevating the sensitivity of gingival epithelial cells to microorganisms. Taken together, these data indicate that Ti ions may be involved in cytotoxicity and inflammation at the interfaces of dental implants and gingival tissue.
Volume 24(7)
Pages 1905-10
Published 2010-10-1
DOI 10.1016/j.tiv.2010.07.023
PII S0887-2333(10)00184-0
PMID 20682337
MeSH Animals Cell Survival / drug effects Cells, Cultured Dose-Response Relationship, Drug Drug Synergism Epithelial Cells / drug effects* Epithelial Cells / pathology Gene Expression Regulation / drug effects Gingiva / cytology Gingiva / drug effects* Gingiva / pathology Lipopolysaccharides / toxicity Mice Mice, Inbred C57BL Mice, Transgenic Monocytes / metabolism Necrosis / chemically induced* RNA, Messenger / metabolism Titanium / administration & dosage Titanium / toxicity*
IF 2.959
Times Cited 32
Human and Animal Cells GE1(RCB1709)