| RRC ID |
38267
|
| 著者 |
Makihira S, Mine Y, Nikawa H, Shuto T, Iwata S, Hosokawa R, Kamoi K, Okazaki S, Yamaguchi Y.
|
| タイトル |
Titanium ion induces necrosis and sensitivity to lipopolysaccharide in gingival epithelial-like cells.
|
| ジャーナル |
Toxicol In Vitro
|
| Abstract |
Gingival epithelial-like cells (GE-1) were cultured and used to examine the cellular responses of gingival tissues to varying concentrations of titanium (Ti) ions. Titanium ions at concentrations of more than 13 ppm significantly decreased the viability of GE-1 cells and increased LDH release from the cells into the supernatant, but had no significant effect on their caspase 3 activity. These data suggest that a high concentration of Ti ions induced necrosis of the GE-1 cells. Titanium ions at a concentration of 5 ppm significantly increased the level of CCL2 mRNA expression in GE-1 cells exposed to lipopolysaccharide derived from Porphyromonas gingivalis in a synergistic manner. Moreover, the mRNA expression levels of TLR-4 and ICAM-1 in GE-1 cells loaded with Ti ions at 9 ppm were significantly enhanced as compared with those in GE-1 cells without Ti stimulation. We suggest that Ti ions are in part responsible for monocyte infiltration in the oral cavity by elevating the sensitivity of gingival epithelial cells to microorganisms. Taken together, these data indicate that Ti ions may be involved in cytotoxicity and inflammation at the interfaces of dental implants and gingival tissue.
|
| 巻・号 |
24(7)
|
| ページ |
1905-10
|
| 公開日 |
2010-10-1
|
| DOI |
10.1016/j.tiv.2010.07.023
|
| PII |
S0887-2333(10)00184-0
|
| PMID |
20682337
|
| MeSH |
Animals
Cell Survival / drug effects
Cells, Cultured
Dose-Response Relationship, Drug
Drug Synergism
Epithelial Cells / drug effects*
Epithelial Cells / pathology
Gene Expression Regulation / drug effects
Gingiva / cytology
Gingiva / drug effects*
Gingiva / pathology
Lipopolysaccharides / toxicity
Mice
Mice, Inbred C57BL
Mice, Transgenic
Monocytes / metabolism
Necrosis / chemically induced*
RNA, Messenger / metabolism
Titanium / administration & dosage
Titanium / toxicity*
|
| IF |
2.959
|
| 引用数 |
32
|
|
WOS 分野
|
TOXICOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
GE1(RCB1709) |
