論文 - 詳細
| RRC ID | 38275 |
|---|---|
| 著者 | Yumita N, Okudaira K, Momose Y, Umemura S. |
| タイトル | Sonodynamically induced apoptosis and active oxygen generation by gallium-porphyrin complex, ATX-70. |
| ジャーナル | Cancer Chemother Pharmacol |
| Abstract |
In this study, we investigated the induction of apoptosis by ultrasound in the presence of the photochemically active gallium-porphyrin complex, 7,12-bis(1-decyloxyethyl)-Ga(III)-3,8,13,17-tetramethyl-porphyrin 2,18-dipropionyl diaspartic acid (ATX-70). HL-60 cells were exposed to ultrasound for up to 3 min in the presence and absence of ATX-70, and the induction of apoptosis was examined by analyzing cell morphology, DNA fragmentation, and caspase-3 activity. Cells treated with 80 μM ATX-70 and ultrasound clearly showed membrane blebbing and cell shrinkage, whereas significant morphologic changes were not observed in cells exposed to either ultrasound or ATX-70 alone. Also, DNA ladder formation and caspase-3 activation were observed in cells treated with both ultrasound and ATX-70 but not in cells treated with ultrasound or ATX-70 alone. In addition, the combination of ATX-70 and the same acoustical arrangement of ultrasound substantially enhanced nitroxide generation by the cells. Sonodynamically induced apoptosis, caspase-3 activation, and nitroxide generation were significantly suppressed by histidine. These results indicate that the combination of ultrasound and ATX-70 induces apoptosis in HL-60 cells. The significant reduction in sonodynamically induced apoptosis, nitroxide generation, and caspase-3 activation by histidine suggests that active species such as singlet oxygen are important in the sonodynamic induction of apoptosis. |
| 巻・号 | 66(6) |
| ページ | 1071-8 |
| 公開日 | 2010-11-1 |
| DOI | 10.1007/s00280-010-1264-6 |
| PMID | 20204369 |
| MeSH | Apoptosis / drug effects* Caspase 3 / drug effects Caspase 3 / metabolism DNA Fragmentation Drug Combinations Electron Spin Resonance Spectroscopy Electrophoresis Enzyme Activation / drug effects Gallium / pharmacology HL-60 Cells / diagnostic imaging* HL-60 Cells / drug effects* Histidine / metabolism Humans Nitrogen Oxides / metabolism Porphyrins / pharmacology* Reactive Oxygen Species / metabolism Ultrasonic Therapy* / methods Ultrasonography |
| IF | 2.967 |
| 引用数 | 44 |
| WOS 分野 | PHARMACOLOGY & PHARMACY ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | HL60(RCB0041) |