| RRC ID |
38305
|
| 著者 |
Furuta H, Kondo Y, Nakahata S, Hamasaki M, Sakoda S, Morishita K.
|
| タイトル |
NDRG2 is a candidate tumor-suppressor for oral squamous-cell carcinoma.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
Oral cancer is one of the most common cancers worldwide, and squamous-cell carcinoma (OSCC) is the most common phenotype of oral cancer. Although patients with OSCC have poor survival rates and a high incidence of metastasis, the molecular mechanisms of OSCC development have not yet been elucidated. This study investigated whether N-myc downstream-regulated gene 2 (NDRG2) contributes to the carcinogenesis of OSCC, as NDRG2 is reported to be a candidate tumor-suppressor gene in a wide variety of cancers. The down-regulation of NDRG2 mRNA, which was dependent on promoter methylation, was seen in the majority of OSCC cases and in several cases of precancerous leukoplakia with dysplasia. Induction of NDRG2 expression in an HSC-3/OSCC cell line significantly inhibited cell proliferation and decreased colony formation ability on soft agar. The majority of OSCC cell lines showed an activation of PI3K/Akt signaling, and enforced expression of NDRG2 in HSC-3 cells decreased the level of phosphorylated Akt at Serine 473 (p-Akt). Immunohistochemical p-Akt staining was detected in 56.5% of the OSCC tumors, and 80.4% of the tumors were negative for NDRG2 staining. Moreover, positive p-Akt staining was inversely correlated with decreased NDRG2 expression in OSCC tumors with moderate to poor differentiation (p<0.005). Therefore, NDRG2 is a candidate tumor-suppressor gene for OSCC development and probably contributes to the tumorigenesis of OSCC partly via the modulation of Akt signaling.
|
| 巻・号 |
391(4)
|
| ページ |
1785-91
|
| 公開日 |
2010-1-22
|
| DOI |
10.1016/j.bbrc.2009.12.156
|
| PII |
S0006-291X(09)02554-6
|
| PMID |
20045673
|
| MeSH |
Carcinoma, Squamous Cell / genetics*
Carcinoma, Squamous Cell / metabolism
Cell Line, Tumor
DNA Methylation
Down-Regulation
Gene Expression Regulation, Neoplastic*
Humans
Mouth Neoplasms / genetics*
Mouth Neoplasms / metabolism
Promoter Regions, Genetic
Proto-Oncogene Proteins c-akt / metabolism
Tumor Suppressor Proteins / genetics*
|
| IF |
2.985
|
| 引用数 |
36
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
|
