論文 - 詳細
RRC ID | 38321 |
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著者 | Kawano M, Thet MM, Makino T, Kushida T, Sakagami H. |
タイトル | DNA microarray analysis of signaling pathway in macrophages stimulated by lignin-carbohydrate complex from lentinus edodes mycelia (LEM) extract. |
ジャーナル | Anticancer Res |
Abstract |
In order to investigate the action point of lignin-carbohydrate complex (Fr4) from Lentinus edodes mycelia extract, DNA microarray analysis was performed, using mouse macrophage-like J774.1 cells. Among seven lignin-carbohydrate complex fractions, Fr4 showed the highest stimulatory activity of tumor necrosis factor production by mouse macrophage-like J774.1 cells, as well as its previously reported anti-HIV activity. Fr4 is composed of lignin precursors such as vanillic acid, syringic acid, p-coumaric acid and ferulic acid, with trace amounts of flavonoids and tannins, and negligible amount of lipopolysaccharides (LPS), confirming the authenticity of Fr4 as a lignin. DNA microarray analysis suggested that Fr4 may affect immune response-related gene expression; however, it may not affect the expression of as many genes as LPS does. |
巻・号 | 30(7) |
ページ | 2567-76 |
公開日 | 2010-7-1 |
PII | 30/7/2567 |
PMID | 20682984 |
MeSH | Animals Cell Line Cluster Analysis Gene Expression / drug effects Lignin / analysis Lignin / isolation & purification Lignin / pharmacology* Lipopolysaccharides / analysis Lipopolysaccharides / pharmacology Macrophages / drug effects* Macrophages / immunology Macrophages / metabolism Mice Oligonucleotide Array Sequence Analysis / methods Principal Component Analysis Shiitake Mushrooms / chemistry* Signal Transduction / drug effects Stimulation, Chemical Tumor Necrosis Factor-alpha / biosynthesis |
IF | 1.994 |
引用数 | 11 |
WOS 分野 | ONCOLOGY |
リソース情報 | |
ヒト・動物細胞 | J774.1 |