Reference - Detail
| RRC ID | 3837 |
|---|---|
| Author | Sasaki S, Mello CC, Shimada A, Nakatani Y, Hashimoto S, Ogawa M, Matsushima K, Gu SG, Kasahara M, Ahsan B, Sasaki A, Saito T, Suzuki Y, Sugano S, Kohara Y, Takeda H, Fire A, Morishita S. |
| Title | Chromatin-associated periodicity in genetic variation downstream of transcriptional start sites. |
| Journal | Science |
| Abstract |
Might DNA sequence variation reflect germline genetic activity and underlying chromatin structure? We investigated this question using medaka (Japanese killifish, Oryzias latipes), by comparing the genomic sequences of two strains (Hd-rR and HNI) and by mapping approximately 37.3 million nucleosome cores from Hd-rR blastulae and 11,654 representative transcription start sites from six embryonic stages. We observed a distinctive approximately 200-base pair (bp) periodic pattern of genetic variation downstream of transcription start sites; the rate of insertions and deletions longer than 1 bp peaked at positions of approximately +200, +400, and +600 bp, whereas the point mutation rate showed corresponding valleys. This approximately 200-bp periodicity was correlated with the chromatin structure, with nucleosome occupancy minimized at positions 0, +200, +400, and +600 bp. These data exemplify the potential for genetic activity (transcription) and chromatin structure to contribute to molding the DNA sequence on an evolutionary time scale. |
| Volume | 323(5912) |
| Pages | 401-4 |
| Published | 2009-1-16 |
| DOI | 10.1126/science.1163183 |
| PII | 1163183 |
| PMID | 19074313 |
| PMC | PMC2757552 |
| MeSH | Animals Base Composition Base Sequence Chromatin / physiology* Chromatin / ultrastructure DNA / chemistry DNA / genetics* DNA Repair Genetic Variation* Genome INDEL Mutation Mutagenesis Mutation Nucleosomes / physiology* Nucleosomes / ultrastructure Oryzias / embryology Oryzias / genetics* Point Mutation Promoter Regions, Genetic Transcription Initiation Site* Transcription, Genetic |
| IF | 41.846 |
| Times Cited | 98 |
| WOS Category | GENETICS & HEREDITY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | F1000Research |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Medaka | Hd-rRII1 (IB178) HNI-II (IB176) |