| RRC ID |
38404
|
| 著者 |
Hayashi K, Ohta H, Kurimoto K, Aramaki S, Saitou M.
|
| タイトル |
Reconstitution of the mouse germ cell specification pathway in culture by pluripotent stem cells.
|
| ジャーナル |
Cell
|
| Abstract |
The generation of properly functioning gametes in vitro requires reconstitution of the multistepped pathway of germ cell development. We demonstrate here the generation of primordial germ cell-like cells (PGCLCs) in mice with robust capacity for spermatogenesis. PGCLCs were generated from embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) through epiblast-like cells (EpiLCs), a cellular state highly similar to pregastrulating epiblasts but distinct from epiblast stem cells (EpiSCs). Reflecting epiblast development, EpiLC induction from ESCs/iPSCs is a progressive process, and EpiLCs highly competent for the PGC fate are a transient entity. The global transcription profiles, epigenetic reprogramming, and cellular dynamics during PGCLC induction from EpiLCs meticulously capture those associated with PGC specification from the epiblasts. Furthermore, we identify Integrin-β3 and SSEA1 as markers that allow the isolation of PGCLCs with spermatogenic capacity from tumorigenic undifferentiated cells. Our findings provide a paradigm for the first step of in vitro gametogenesis.
|
| 巻・号 |
146(4)
|
| ページ |
519-32
|
| 公開日 |
2011-8-19
|
| DOI |
10.1016/j.cell.2011.06.052
|
| PII |
S0092-8674(11)00771-9
|
| PMID |
21820164
|
| MeSH |
Animals
Cell Culture Techniques
Cell Differentiation*
Cell Proliferation
Embryonic Stem Cells / cytology
Embryonic Stem Cells / metabolism
Gene Expression Profiling
Germ Cells / cytology
Germ Cells / metabolism
Germ Layers / cytology
Germ Layers / metabolism
Induced Pluripotent Stem Cells / cytology
Induced Pluripotent Stem Cells / metabolism
Mice
Pluripotent Stem Cells / cytology*
Pluripotent Stem Cells / metabolism*
Spermatogenesis
|
| IF |
38.637
|
| 引用数 |
602
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
iPS-MEF-Ng-20D-17(APS0001)
iPS-MEF-Ng-178B-5(APS0002)
iPS-MEF-Ng-492B-4(APS0004) |
