論文 - 詳細
| RRC ID | 38404 |
|---|---|
| 著者 | Hayashi K, Ohta H, Kurimoto K, Aramaki S, Saitou M. |
| タイトル | Reconstitution of the mouse germ cell specification pathway in culture by pluripotent stem cells. |
| ジャーナル | Cell |
| Abstract |
The generation of properly functioning gametes in vitro requires reconstitution of the multistepped pathway of germ cell development. We demonstrate here the generation of primordial germ cell-like cells (PGCLCs) in mice with robust capacity for spermatogenesis. PGCLCs were generated from embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) through epiblast-like cells (EpiLCs), a cellular state highly similar to pregastrulating epiblasts but distinct from epiblast stem cells (EpiSCs). Reflecting epiblast development, EpiLC induction from ESCs/iPSCs is a progressive process, and EpiLCs highly competent for the PGC fate are a transient entity. The global transcription profiles, epigenetic reprogramming, and cellular dynamics during PGCLC induction from EpiLCs meticulously capture those associated with PGC specification from the epiblasts. Furthermore, we identify Integrin-β3 and SSEA1 as markers that allow the isolation of PGCLCs with spermatogenic capacity from tumorigenic undifferentiated cells. Our findings provide a paradigm for the first step of in vitro gametogenesis. |
| 巻・号 | 146(4) |
| ページ | 519-32 |
| 公開日 | 2011-8-19 |
| DOI | 10.1016/j.cell.2011.06.052 |
| PII | S0092-8674(11)00771-9 |
| PMID | 21820164 |
| MeSH | Animals Cell Culture Techniques Cell Differentiation* Cell Proliferation Embryonic Stem Cells / cytology Embryonic Stem Cells / metabolism Gene Expression Profiling Germ Cells / cytology Germ Cells / metabolism Germ Layers / cytology Germ Layers / metabolism Induced Pluripotent Stem Cells / cytology Induced Pluripotent Stem Cells / metabolism Mice Pluripotent Stem Cells / cytology* Pluripotent Stem Cells / metabolism* Spermatogenesis |
| IF | 38.637 |
| 引用数 | 602 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 194 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 19.0 |
| リソース情報 | |
| ヒト・動物細胞 | iPS-MEF-Ng-20D-17(APS0001) iPS-MEF-Ng-178B-5(APS0002) iPS-MEF-Ng-492B-4(APS0004) |