| RRC ID |
38463
|
| 著者 |
Suzuki K, Sun R, Origuchi M, Kanehira M, Takahata T, Itoh J, Umezawa A, Kijima H, Fukuda S, Saijo Y.
|
| タイトル |
Mesenchymal stromal cells promote tumor growth through the enhancement of neovascularization.
|
| ジャーナル |
Mol Med
|
| Abstract |
Mesenchymal stromal cells (MSCs), also called mesenchymal stem cells, migrate and function as stromal cells in tumor tissues. The effects of MSCs on tumor growth are controversial. In this study, we showed that MSCs increase proliferation of tumor cells in vitro and promote tumor growth in vivo. We also further analyzed the mechanisms that underlie these effects. For use in in vitro and in vivo experiments, we established a bone marrow-derived mesenchymal stromal cell line from cells isolated in C57BL/6 mice. Effects of murine MSCs on tumor cell proliferation in vitro were analyzed in a coculture model with B16-LacZ cells. Both coculture with MSCs and treatment with MSC-conditioned media led to enhanced growth of B16-LacZ cells, although the magnitude of growth stimulation in cocultured cells was greater than that of cells treated with conditioned media. Co-injection of B16-LacZ cells and MSCs into syngeneic mice led to increased tumor size compared with injection of B16-LacZ cells alone. Identical experiments using Lewis lung carcinoma (LLC) cells instead of B16-LacZ cells yielded similar results. Consistent with a role for neovascularization in MSC-mediated tumor growth, tumor vessel area was greater in tumors resulting from co-injection of B16-LacZ cells or LLCs with MSCs than in tumors induced by injection of cancer cells alone. Co-injected MSCs directly supported the tumor vasculature by localizing close to vascular walls and by expressing an endothelial marker. Furthermore, secretion of leukemia inhibitory factor, macrophage colony-stimulating factor, macrophage inflammatory protein-2 and vascular endothelial growth factor was increased in cocultures of MSCs and B16-LacZ cells compared with B16-LacZ cells alone. Together, these results indicate that MSCs promote tumor growth both in vitro and in vivo and suggest that tumor promotion in vivo may be attributable in part to enhanced angiogenesis.
|
| 巻・号 |
17(7-8)
|
| ページ |
579-87
|
| 公開日 |
2011-1-1
|
| DOI |
10.2119/molmed.2010.00157
|
| PII |
molmed.2010.00157
|
| PMID |
21424106
|
| PMC |
PMC3146617
|
| MeSH |
Animals
Antigens, CD34 / metabolism
Cell Line, Tumor
Cell Proliferation
Cells, Cultured
Chemokine CXCL2 / metabolism
Coculture Techniques
Female
Flow Cytometry
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism
Macrophage Colony-Stimulating Factor / metabolism
Mesenchymal Stem Cell Transplantation
Mesenchymal Stem Cells / cytology*
Mesenchymal Stem Cells / metabolism
Mice
Mice, Transgenic
Microscopy, Confocal
Neoplasm Transplantation
Neoplasms, Experimental / blood supply
Neoplasms, Experimental / metabolism
Neoplasms, Experimental / pathology*
Neovascularization, Pathologic / metabolism
Neovascularization, Pathologic / pathology*
Tumor Burden
Vascular Endothelial Growth Factor A / metabolism
|
| IF |
4.096
|
| 引用数 |
134
|
|
WOS 分野
|
MEDICINE, RESEARCH & EXPERIMENTAL
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
|
