RRC ID 385
Author Kikuchi Y, Verkade H, Reiter JF, Kim CH, Chitnis AB, Kuroiwa A, Stainier DY.
Title Notch signaling can regulate endoderm formation in zebrafish.
Journal Dev. Dyn.
Abstract Early in vertebrate development, the processes of gastrulation lead to the formation of the three germ layers: ectoderm, mesoderm, and endoderm. The mechanisms leading to the segregation of the endoderm and mesoderm are not well understood. In mid-blastula stage zebrafish embryos, single marginal cells can give rise to both endoderm and mesoderm (reviewed by Warga and Stainier [2002] The guts of endoderm formation. In: Solnica-Krezel L, editor. Pattern formation in zebrafish. Berlin: Springer-Verlag. p 28-47). By the late blastula stage, however, single marginal cells generally give rise to either endoderm or mesoderm. To investigate this segregation of the blastoderm into cells with either endodermal or mesodermal fates, we analyzed the role of Notch signaling in this process. We show that deltaC, deltaD, and notch1 are expressed in the marginal domain of blastula stage embryos and that this expression is dependent on Nodal signaling. Activation of Notch signaling from an early stage leads to a reduction of endodermal cells, as assessed by sox17 and foxA2 expression. We further find that this reduction in endoderm formation by the activation of Notch signaling is preceded by a reduction in the expression of bonnie and clyde (bon) and faust/gata5, two genes necessary for endoderm formation (Reiter et al. [1999] Genes Dev 13:2983-2995; Reiter et al. [2001] Development 128:125-135; Kikuchi et al. [2001] Genes Dev 14:1279-1289). However, activation of Notch signaling in bon mutant embryos leads to a further reduction in endodermal cells, also arguing for a bon-independent role for Notch signaling in endoderm formation. Altogether, these results suggest that Notch signaling plays a role in the formation of the endoderm, possibly in its segregation from the mesoderm.
Volume 229(4)
Pages 756-62
Published 2004-4
DOI 10.1002/dvdy.10483
PMID 15042699
MeSH Animals DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism Embryo, Nonmammalian / metabolism Endoderm / metabolism* GATA5 Transcription Factor Gastrula / metabolism Gene Expression Hepatocyte Nuclear Factor 3-beta High Mobility Group Proteins / genetics High Mobility Group Proteins / metabolism Homeodomain Proteins / genetics Homeodomain Proteins / metabolism Intracellular Signaling Peptides and Proteins Membrane Proteins / genetics Membrane Proteins / metabolism Nerve Tissue Proteins / genetics Nerve Tissue Proteins / metabolism Nuclear Proteins / genetics Nuclear Proteins / metabolism Receptor, Notch1 Receptors, Cell Surface / genetics Receptors, Cell Surface / metabolism Receptors, Cell Surface / physiology* SOXF Transcription Factors Signal Transduction* Transcription Factors / genetics Transcription Factors / metabolism Transcription Factors / physiology* Xenopus Proteins* Zebrafish / embryology* Zebrafish / metabolism Zebrafish Proteins / genetics Zebrafish Proteins / metabolism
IF 2.852
Times Cited 35
Zebrafish ?