論文 - 詳細
| RRC ID | 38507 |
|---|---|
| 著者 | Kida K, Nakajima M, Mohri T, Oda Y, Takagi S, Fukami T, Yokoi T. |
| タイトル | PPARα is regulated by miR-21 and miR-27b in human liver. |
| ジャーナル | Pharm Res |
| Abstract |
PURPOSE:Peroxisome proliferator-activated receptor α (PPARα) is an important transcriptional factor that regulates genes encoding endo/xenobiotic enzymes and lipid metabolizing enzymes. In this study, we investigated whether microRNAs (miRNAs) are involved in the regulation of PPARα in human liver. METHODS:Precursor or antisense oligonucleotide for miR-21 or miR-27b was transfected into HuH7 cells; expression of PPARα and acyl-CoA synthetase M2B was determined by Western blot and real-time RT-PCR. Luciferase assay was performed to identify the functional miRNA recognition element (MRE). Expression levels of PPARα, miR-21, and miR-27b in a panel of 24 human livers were determined. RESULTS:The overexpression and inhibition of miR-21 or miR-27b in HuH7 cells significantly decreased and increased the PPARα protein level, respectively, but not PPARα mRNA level. The miRNA-dependent regulation of PPARα affected the expression of its downstream gene. Luciferase assay identified a functional MRE for miR-21 in the 3'-untranslated region of PPARα. In human livers, the PPARα protein levels were not correlated with PPARα mRNA, but inversely correlated with the miR-21 levels, suggesting a substantial impact of miR-21, although the contribution of miR-27b could not be ruled out. CONCLUSIONS:We found that PPARα in human liver is regulated by miRNAs. |
| 巻・号 | 28(10) |
| ページ | 2467-76 |
| 公開日 | 2011-10-1 |
| DOI | 10.1007/s11095-011-0473-y |
| PMID | 21562928 |
| MeSH | 3' Untranslated Regions Cell Line, Transformed Cell Line, Tumor Gene Expression Regulation* HEK293 Cells HeLa Cells Hep G2 Cells Humans Liver / metabolism Liver / physiology* MicroRNAs / genetics* MicroRNAs / metabolism PPAR alpha / genetics* PPAR alpha / metabolism RNA, Messenger / genetics Transfection |
| IF | 3.242 |
| 引用数 | 87 |
| WOS 分野 | PHARMACOLOGY & PHARMACY CHEMISTRY, MULTIDISCIPLINARY |
| リソース情報 | |
| ヒト・動物細胞 | HuH-7(RCB1366) Hep G2 |