論文 - 詳細
| RRC ID | 38571 |
|---|---|
| 著者 | Tonoike Y, Matsushita K, Tomonaga T, Katada K, Tanaka N, Shimada H, Nakatani Y, Okamoto Y, Nomura F. |
| タイトル | Adhesion molecule periplakin is involved in cellular movement and attachment in pharyngeal squamous cancer cells. |
| ジャーナル | BMC Cell Biol |
| Abstract |
BACKGROUND:We previously reported that periplakin (PPL) is downregulated in human esophageal cancer tissues compared to the adjacent non-cancer epithelium. Thus PPL could be a useful marker for detection of early esophageal cancer and evaluation of tumor progression, but largely remains unknown in this field. To investigate PPL involvement in carcinogenesis, tumor progression, cellular movement or attachment activity, siRNAs against PPL were transfected into pharyngeal squamous cancer cell lines and their effects on cellular behaviours were examined. RESULTS:PPL knockdown appeared to decrease tumor cell growth together with G2/M phase accumulation in cells attached to a culture dish. However, the extent of cell growth suppression, evaluated by the number of cells attached to the culture dish, was too distinctive to be explained only by cell cycle delay. Importantly, PPL knockdown suppressed cellular movement and attachment to the culture dish accompanied by decreased pAktSer473 phosphorylation. Additionally, LY294002, a PI3K inhibitor that dephosphorylates pAktSer473, significantly suppressed D562 cell migration. Thus PPL potentially engages in cellular movement al least partly via the PI3K/Akt axis. CONCLUSIONS:PPL knockdown is related to reduced cellular movement and attachment activity in association with PI3K/Akt axis suppression, rather than malignant progression in pharyngeal cancer cells. |
| 巻・号 | 12 |
| ページ | 41 |
| 公開日 | 2011-9-27 |
| DOI | 10.1186/1471-2121-12-41 |
| PII | 1471-2121-12-41 |
| PMID | 21951621 |
| PMC | PMC3195110 |
| MeSH | Carcinoma, Squamous Cell / genetics Carcinoma, Squamous Cell / metabolism* Carcinoma, Squamous Cell / pathology Cell Adhesion* / genetics Cell Cycle / genetics Cell Growth Processes / genetics Cell Line, Tumor Cell Movement* / drug effects Cell Movement* / genetics Chromones / pharmacology Humans Morpholines / pharmacology Pharyngeal Neoplasms / genetics Pharyngeal Neoplasms / metabolism* Pharyngeal Neoplasms / pathology Phosphoinositide-3 Kinase Inhibitors Phosphorylation / drug effects Plakins / genetics Plakins / metabolism* Proto-Oncogene Proteins c-akt / metabolism RNA, Small Interfering / genetics |
| IF | 3.066 |
| 引用数 | 12 |
| WOS 分野 | CELL BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | TE-4(RCB2097) TE-9(RCB1988) TE-11(RCB2100) |