論文 - 詳細
| RRC ID | 38584 |
|---|---|
| 著者 | Tang X, Xing Z, Tang H, Liang L, Zhao M. |
| タイトル | Human cell-death-inducing DFF45-like effector C induces apoptosis via caspase-8. |
| ジャーナル | Acta Biochim Biophys Sin (Shanghai) |
| Abstract |
Human cell-death-inducing DNA-fragmentation-factor (DFF45)-like effector C (CIDEC) is a potent apoptotic inducer. Previous studies have indicated that the Fat-specific protein 27 (Fsp27), a mouse homolog of CIDEC, induces apoptosis via caspase-3, -7, and -9 and triggers the release of cytochrome c from mitochondria, which implies that the mitochondrial pathway is involved in Fsp27-induced apoptosis. In the current study, we found that CIDEC-induced apoptosis was mediated by caspase-8. The caspase inhibitor assay showed that CIDEC-induced apoptosis was dramatically reduced in the presence of the general caspase inhibitor, the caspase-3 inhibitor, and the caspase-8 inhibitor, whereas the caspase-9 inhibitor only weakly inhibited CIDEC-induced apoptosis. These results confirmed that the activation of caspase-3 and caspase-8 were involved in CIDEC-induced apoptosis. Moreover, in caspase-3- or caspase-8-deficient cells, CIDEC-induced apoptosis were dramatically decreased, which demonstrated that CIDEC-induced apoptosis might require the activation of caspase-3 and caspase-8. Because caspase-8 in general is a key effecter of death-receptor pathway and activated by Fas-Associated protein with Death Domain (FADD), we examined whether FADD was involved in CIDEC-induced apoptosis. Our results demonstrated that CIDEC-induced apoptosis was independent of FADD, suggesting that CIDEC-induced apoptosis might be in a death-receptor-independent, caspase-8-dependent manner. It was also found that the region of amino acid 168-200 in carboxyl domain of CIDEC was critical for its crucial pro-apoptotic function. |
| 巻・号 | 43(10) |
| ページ | 779-86 |
| 公開日 | 2011-10-1 |
| DOI | 10.1093/abbs/gmr073 |
| PII | gmr073 |
| PMID | 21865223 |
| MeSH | Amino Acid Sequence Animals Apoptosis* Apoptosis Regulatory Proteins Caspase 8 / metabolism* Cell Line Cell Line, Tumor DNA Fragmentation Enzyme Activation Fas-Associated Death Domain Protein / metabolism Humans Mice Molecular Sequence Data Proteins / chemistry Proteins / metabolism* |
| IF | 2.836 |
| 引用数 | 8 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | |