論文 - 詳細
| RRC ID | 386 |
|---|---|
| 著者 | Reim G, Mizoguchi T, Stainier DY, Kikuchi Y, Brand M. |
| タイトル | The POU domain protein spg (pou2/Oct4) is essential for endoderm formation in cooperation with the HMG domain protein casanova. |
| ジャーナル | Dev Cell |
| Abstract |
The gastrulating vertebrate embryo develops three germlayers: ectoderm, mesoderm, and endoderm. Zebrafish endoderm differentiation starts with the activation of sox17 by casanova (cas). We report that spg (pou2/Oct4) is essential for endoderm formation. Embryos devoid of maternal and zygotic spg function (MZspg) lack endodermal precursors. Cell transplantations show that spg acts in early endodermal precursors, and cas mRNA-injection into MZspg embryos does not restore endoderm development. spg and cas together are both necessary and sufficient to activate endoderm development, and stimulate expression of a sox17 promoter-luciferase reporter. Endoderm and mesoderm derive from a common origin, mesendoderm. We propose that Spg and Cas commit mesendodermal precursors to an endodermal fate. The joint control of endoderm formation by spg and cas suggests that the endodermal germlayer may be a tissue unit with distinct genetic control, thus adding genetic support to the germlayer concept in metazoan development. |
| 巻・号 | 6(1) |
| ページ | 91-101 |
| 公開日 | 2004-1-1 |
| DOI | 10.1016/s1534-5807(03)00396-4 |
| PII | S1534-5807(03)00396-4 |
| PMID | 14723850 |
| MeSH | Animals Body Patterning / genetics Cell Differentiation / genetics Cell Lineage / genetics DNA-Binding Proteins / genetics Embryo, Nonmammalian / cytology Embryo, Nonmammalian / embryology* Embryo, Nonmammalian / metabolism Endoderm / cytology Endoderm / metabolism* Female Gene Expression Regulation, Developmental / genetics High Mobility Group Proteins / genetics High Mobility Group Proteins / metabolism* Male Octamer Transcription Factor-3 Promoter Regions, Genetic / genetics RNA, Messenger / pharmacology SOX Transcription Factors SOXF Transcription Factors Stem Cells / cytology Stem Cells / metabolism Transcription Factors / deficiency* Transcription Factors / genetics Transcription Factors / metabolism* Zebrafish / embryology* Zebrafish / metabolism Zebrafish Proteins* Zygote / cytology Zygote / metabolism |
| IF | 10.092 |
| 引用数 | 88 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゼブラフィッシュ | |