RRC ID 386
著者 Reim G, Mizoguchi T, Stainier DY, Kikuchi Y, Brand M.
タイトル The POU domain protein spg (pou2/Oct4) is essential for endoderm formation in cooperation with the HMG domain protein casanova.
ジャーナル Dev Cell
Abstract The gastrulating vertebrate embryo develops three germlayers: ectoderm, mesoderm, and endoderm. Zebrafish endoderm differentiation starts with the activation of sox17 by casanova (cas). We report that spg (pou2/Oct4) is essential for endoderm formation. Embryos devoid of maternal and zygotic spg function (MZspg) lack endodermal precursors. Cell transplantations show that spg acts in early endodermal precursors, and cas mRNA-injection into MZspg embryos does not restore endoderm development. spg and cas together are both necessary and sufficient to activate endoderm development, and stimulate expression of a sox17 promoter-luciferase reporter. Endoderm and mesoderm derive from a common origin, mesendoderm. We propose that Spg and Cas commit mesendodermal precursors to an endodermal fate. The joint control of endoderm formation by spg and cas suggests that the endodermal germlayer may be a tissue unit with distinct genetic control, thus adding genetic support to the germlayer concept in metazoan development.
巻・号 6(1)
ページ 91-101
公開日 2004-1-1
DOI 10.1016/s1534-5807(03)00396-4
PII S1534-5807(03)00396-4
PMID 14723850
MeSH Animals Body Patterning / genetics Cell Differentiation / genetics Cell Lineage / genetics DNA-Binding Proteins / genetics Embryo, Nonmammalian / cytology Embryo, Nonmammalian / embryology* Embryo, Nonmammalian / metabolism Endoderm / cytology Endoderm / metabolism* Female Gene Expression Regulation, Developmental / genetics High Mobility Group Proteins / genetics High Mobility Group Proteins / metabolism* Male Octamer Transcription Factor-3 Promoter Regions, Genetic / genetics RNA, Messenger / pharmacology SOX Transcription Factors SOXF Transcription Factors Stem Cells / cytology Stem Cells / metabolism Transcription Factors / deficiency* Transcription Factors / genetics Transcription Factors / metabolism* Zebrafish / embryology* Zebrafish / metabolism Zebrafish Proteins* Zygote / cytology Zygote / metabolism
IF 10.092
引用数 88
WOS 分野 DEVELOPMENTAL BIOLOGY CELL BIOLOGY
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