RRC ID 38629
Author Tanaka Y, Tsuyuki M, Itaya-Hironaka A, Inada Y, Yoshihara K, Kamiya T.
Title Retinoic acid-induced differentiation-specific, C-kinase-dependent phosphorylation of cytosolic 44 and 32 kDa proteins in HL-60 cells.
Journal Biochem Biophys Res Commun
Abstract The effect of various differentiation-inducers on the activity of Ca2+, phospholipid-dependent protein kinase (C-kinase) activity and endogenous protein phosphorylation by the kinase were examined in the extracts of HL-60 cells. Although all of the inducers, retinoic acid, dibutyryl cAMP, nicotinamide, dimethylsulfoxide, and 3-aminobenzamide increased the cytosolic C-kinase activity accompanied with the differentiation into mature myelocytes, only retinoic acid markedly enhanced Ca2+, phospholipid-dependent phosphorylation of 44 and 32 kDa proteins in the cytosol. These results suggest that the differentiation pathway induced by retinoic acid is different from the pathways induced by other inducers.
Volume 168(3)
Pages 1253-60
Published 1990-5-16
DOI 10.1016/0006-291x(90)91163-m
PII 0006-291X(90)91163-M
PMID 2161220
MeSH Benzamides / pharmacology Bucladesine / pharmacology Calcium / metabolism Cell Differentiation / drug effects* Cell Extracts Cells, Cultured Cytosol / enzymology Dimethyl Sulfoxide / pharmacology Electrophoresis, Polyacrylamide Gel Humans Molecular Weight Niacinamide / pharmacology Phosphorylation Protein Kinase C / metabolism* Proteins / metabolism* Tretinoin / pharmacology*
IF 2.705
Times Cited 16
WOS Category BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Human and Animal Cells