RRC ID 38637
著者 Sato T, Hong MH, Jin CH, Ishimi Y, Udagawa N, Shinki T, Abe E, Suda T.
タイトル The specific production of the third component of complement by osteoblastic cells treated with 1 alpha,25-dihydroxyvitamin D3.
ジャーナル FEBS Lett
Abstract A 190 kDa protein was purified from conditioned media of mouse marrow-derived stromal cell (ST2) cultures treated with 1 alpha,25-dihydroxyvitamin D3 (1 alpha,25(OH)2D3) and identified as the third component of mouse complement (C3). Northern and Western blot analysis revealed that the production of C3 by ST2 and primary osteoblastic cells was strictly dependent on 1 alpha,25(OH)2D3, but the production by hepatocytes was not. Adding 1 alpha,25(OH)2D3 together with mouse C3 antibody to bone marrow cultures greatly inhibited the formation of tartrate-resistant acid phosphatase (TRAP)-positive osteoclast-like multinucleated cells. Adding C3 alone induced no TRAP-positive cell formation. These results suggest that, in bone tissues, C3 is specifically produced by osteoblasts in response to 1 alpha,25(OH)2D3 and somehow involved in inducing differentiation of bone marrow cells into osteoclasts in concert with other factors produced by osteoblasts in response to 1 alpha,25(OH)2D3.
巻・号 285(1)
ページ 21-4
公開日 1991-7-8
DOI 10.1016/0014-5793(91)80715-f
PII 0014-5793(91)80715-F
PMID 2065778
MeSH Acid Phosphatase / biosynthesis Amino Acid Sequence Animals Blotting, Northern Blotting, Western Bone Marrow Cells Calcitriol / pharmacology* Cell Differentiation / drug effects Cell Line Cells, Cultured Complement C3 / biosynthesis* Complement C3 / immunology Electrophoresis, Polyacrylamide Gel Liver / cytology Mice Molecular Sequence Data Osteoblasts / drug effects Osteoblasts / immunology Osteoblasts / metabolism*
IF 3.057
引用数 41
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
ヒト・動物細胞 ST2(RCB0224)