Reference - Detail
| RRC ID | 38667 |
|---|---|
| Author | Wang ZQ, Hemken P, Menton D, Gluck S. |
| Title | Expression of vacuolar H(+)-ATPase in mouse osteoclasts during in vitro differentiation. |
| Journal | Am J Physiol |
| Abstract |
Osteoclasts express high levels of a vacuolar H(+)-adenosinetriphosphatase (H(+)-ATPase) on the ruffled membrane which they employ to dissolve bone mineral by acidifying their site of attachment on bone. The factors that control amplification of H(+)-ATPase during osteoclast differentiation are poorly understood. We examined the expression of vacuolar H(+)-ATPase in a cell culture system in which mouse spleen cells can be induced to differentiate into osteoclasts by coculture with a mouse bone marrow stromal cell line. We found that the coculture system produced active osteoclasts, identified as multinucleated cells with staining for tartrate-resistant acid phosphatase activity that formed genuine resorption pits in bone. These cells developed high levels of H(+)-ATPase expression in culture, and omission of dexamethasone or 1 alpha,25-dihydroxyvitamin D3 from the coculture system each partially suppressed the expression of H(+)-ATPase. The results demonstrate that the spleen and PA6 cell coculture system may be useful for investigating the factors that control the induction of H(+)-ATPase amplification that occurs during osteoclast differentiation. |
| Volume | 263(2 Pt 2) |
| Pages | F277-83 |
| Published | 1992-8-1 |
| DOI | 10.1152/ajprenal.1992.263.2.F277 |
| PMID | 1387291 |
| MeSH | Acid Phosphatase / metabolism Animals Bone Resorption Cell Differentiation Cells, Cultured Cytological Techniques Dexamethasone / pharmacology Dihydroxycholecalciferols / pharmacology Drug Resistance Genetic Markers Mice Microscopy, Electron, Scanning Osteoclasts / cytology Osteoclasts / enzymology* Osteoclasts / ultrastructure Phenotype Proton-Translocating ATPases / metabolism* Spleen / cytology Tartrates / pharmacology Vacuoles / enzymology* |
| IF | 3.485 |
| Times Cited | 11 |
| WOS Category | PHYSIOLOGY |
| Resource | |
| Human and Animal Cells | ST2(RCB0224) |