Reference - Detail
| RRC ID | 38675 |
|---|---|
| Author | Fujimoto N, Zhang J, Iwata K, Shinya T, Okada Y, Hayakawa T. |
| Title | A one-step sandwich enzyme immunoassay for tissue inhibitor of metalloproteinases-2 using monoclonal antibodies. |
| Journal | Clin Chim Acta |
| Abstract |
A one-step sandwich enzyme immunoassay system was developed with a pair of monoclonal antibodies against two individual oligopeptides prepared from the amino acid sequence of the human tissue inhibitor of metalloproteinases-2 (TIMP-2). The assay system consisting of two simultaneous immunoreactions used a solid phase monoclonal antibody and a horse-radish peroxidase-labeled monoclonal antibody. The system detected a free form of TIMP-2 and that complexed with active forms of matrix metalloproteinases (MMPs) giving a different sensitivity for each MMP but not TIMP-2 complexed with the precursor of 72 kDa gelatinase/type IV collagenase (MMP-2). The sensitivity of the system was 1.6 microgram/l (16 pg/assay) and linearity was obtained between 6.3 and 50 micrograms/l (63-500 pg/assay). TIMP-2 levels in the sera of 20 patients with rheumatoid arthritis (68 +/- 25 micrograms/l, mean +/- S.D.) and 13 patients with hepatocellular carcinoma (76 +/- 46 micrograms/l) were significantly higher (P < 0.05) than those of 18 normal subjects (5.6 +/- 7.4 micrograms/l). In contrast, the levels in the sera of 10 patients with gastric cancer (45 +/- 18 micrograms/l) and 7 patients with cancer of the uterus (36 +/- 13 micrograms/l) were significantly lower (P < 0.05 or P < 0.01) than those of normal subjects. Immunoreactivity analyses suggested that the precursor of MMP-2 in normal sera exists in a complexed form with TIMP-2 by interacting with the C-terminal domain of TIMP-2. |
| Volume | 220(1) |
| Pages | 31-45 |
| Published | 1993-10-29 |
| DOI | 10.1016/0009-8981(93)90004-n |
| PII | 0009-8981(93)90004-N |
| PMID | 8287559 |
| MeSH | Amino Acid Sequence Animals Antibodies, Monoclonal* Cells, Cultured Chromatography, Gel Female Humans Immunoenzyme Techniques* Metalloendopeptidases / antagonists & inhibitors* Mice Mice, Inbred BALB C Molecular Sequence Data Placenta / chemistry Pregnancy Proteins / analysis* Proteins / isolation & purification Sensitivity and Specificity Tissue Inhibitor of Metalloproteinase-2 |
| IF | 2.615 |
| Times Cited | 110 |
| WOS Category | MEDICAL LABORATORY TECHNOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 10 |
| Altmetric score changes over past 6months | 3.0 |
| Resource | |
| Human and Animal Cells | NB1RGB(RCB0222) |