RRC ID 38684
著者 Kobayashi H, Mizuki T, Tsutsui M, Minami K, Yanagihara N, Yuhi T, Izumi F.
タイトル Receptors for C-type natriuretic peptide in cultured rat glial cells.
ジャーナル Brain Res
Abstract To characterize sites of action of C-type natriuretic peptide (CNP) in the glial cells, the effect of CNP on cGMP accumulation and the binding of [125I]CNP in rat astrocyte RCR-1 cells were studied. CNP stimulated cGMP accumulation in the cells from 10(-9) M in a dose-dependent manner, but ANP (atrial natriuretic peptide) had a negligible effect on cGMP accumulation in the cells. [125I]CNP was bound to the cells and its Kd value was 2 orders of magnitude lower than that of the ED50 value for stimulation of cGMP accumulation in the cells. Not only CNP but also ANP displaced [125I]CNP binding to the cells. These results suggest that RCR-1 cells have a B-receptor which contains a guanylate cyclase domain and is preferentially activated by CNP, and that they also have a C-receptor which does not contain a guanylate cyclase domain that reacts with both ANP and CNP.
巻・号 617(1)
ページ 163-6
公開日 1993-7-16
DOI 10.1016/0006-8993(93)90630-6
PII 0006-8993(93)90630-6
PMID 8104082
MeSH Animals Astrocytes / chemistry* Astrocytes / metabolism Atrial Natriuretic Factor / pharmacology Cell Line Cyclic GMP / metabolism Guanylate Cyclase / analysis* Rats Receptors, Atrial Natriuretic Factor / analysis*
IF 2.733
引用数 19
WOS 分野 NEUROSCIENCES
リソース情報
ヒト・動物細胞 RCR-1(RCB0075)