| RRC ID |
38699
|
| Author |
Hu ZQ, Asano K, Yamazaki T, Shimamura T.
|
| Title |
Effect of lipopolysaccharide on mouse mast cell induction by a splenic cell culture system.
|
| Journal |
Infect Immun
|
| Abstract |
We have previously reported a method of mast cell induction by long-term culture of mouse spleen cells without using exogenous mast cell growth factor (Z.-Q. Hu, T. Yoshida, and T. Shimamura, J. Immunol. Methods 149:173, 1992). Supernatants recovered from the long-term cultures contain endogenous interleukin 3 and soluble stem cell factor. These were assessed by the capacity of the recovered supernatants to foster the growth of a mast cell growth factor-dependent cell line and by neutralizing antibodies. Besides the soluble factors, cell-to-cell contacts mediated by membrane stem cell factor on splenic stromal cells and c-Kit receptors on mast cells also affect mast cell induction. Different lots of fetal calf serum (FCS) were examined to determine a possible trigger for cytokine production. FCS can be divided into mast cell-inducible and noninducible sera by this process. However, not all FCS lots contain mast cell growth factor. The mast cell-inducible lots contain lipopolysaccharide (LPS) confirmed by a Limulus assay. Polymyxin B can neutralize the mast cell induction activity. Non-mast cell-inducible FCS can be converted to inducible FCS by adding exogenous LPS. The results indicate that LPS as a trigger of cytokine production is responsible for mast cell induction.
|
| Volume |
62(9)
|
| Pages |
3844-9
|
| Published |
1994-9-1
|
| DOI |
10.1128/iai.62.9.3844-3849.1994
|
| PMID |
7520422
|
| PMC |
PMC303039
|
| MeSH |
Animals
Cattle
Cell Division
Cells, Cultured
Fetal Blood / physiology
Hematopoietic Cell Growth Factors / physiology
Interleukin-3 / physiology
Lipopolysaccharides / pharmacology*
Male
Mast Cells / drug effects*
Mast Cells / physiology
Mice
Mice, Inbred C3H
Polymyxin B / pharmacology
Stem Cell Factor
|
| IF |
3.201
|
| Times Cited |
14
|
|
WOS Category
|
INFECTIOUS DISEASES
IMMUNOLOGY
|
| Resource |
| Human and Animal Cells |
IC-2(RCB0102) |
