RRC ID |
38740
|
著者 |
Nabeshima K, Kishi J, Kurogi T, Komada N, Kataoka H, Okada Y, Koono M.
|
タイトル |
Stimulation of TIMP-1 and metalloproteinase production in co-cultures of human tumor cells and human fibroblasts.
|
ジャーナル |
Cancer Lett
|
Abstract |
The co-cultures of five different human tumor cell lines with human normal fibroblasts significantly stimulated the production of tissue inhibitors of metalloproteinases-1 (TIMP-1) when compared to cultures of individual cells. In the co-culture of T24 human urinary bladder carcinoma cells and CCD18 human fibroblasts, production of both TIMP-1 and metalloproteinases was stimulated, and the stimulatory effects were dependent on the cellular ratio between the fibroblasts and carcinoma cells. On day 6 of culture, collagenase and stromelysin were stimulated at a ratio of CCD18 fibroblasts to T24 cells of 1:0.1, while the maximum TIMP-1 production occurred at a ratio of 1:1. Thus, the cellular ratio in the interaction of carcinoma cells with host fibroblasts affects the production of TIMP-1 and metalloproteinases and hence modulates the balance between them.
|
巻・号 |
78(1-3)
|
ページ |
133-40
|
公開日 |
1994-4-1
|
DOI |
10.1016/0304-3835(94)90042-6
|
PII |
0304-3835(94)90042-6
|
PMID |
8180955
|
MeSH |
Cells, Cultured
Culture Media
Enzyme Induction
Glycoproteins / biosynthesis*
Humans
Metalloendopeptidases / metabolism*
Tissue Inhibitor of Metalloproteinases
Tumor Cells, Cultured / enzymology*
|
IF |
7.36
|
引用数 |
9
|
WOS 分野
|
ONCOLOGY
|
リソース情報 |
ヒト・動物細胞 |
T24(RCB0431) |