RRC ID 38740
著者 Nabeshima K, Kishi J, Kurogi T, Komada N, Kataoka H, Okada Y, Koono M.
タイトル Stimulation of TIMP-1 and metalloproteinase production in co-cultures of human tumor cells and human fibroblasts.
ジャーナル Cancer Lett
Abstract The co-cultures of five different human tumor cell lines with human normal fibroblasts significantly stimulated the production of tissue inhibitors of metalloproteinases-1 (TIMP-1) when compared to cultures of individual cells. In the co-culture of T24 human urinary bladder carcinoma cells and CCD18 human fibroblasts, production of both TIMP-1 and metalloproteinases was stimulated, and the stimulatory effects were dependent on the cellular ratio between the fibroblasts and carcinoma cells. On day 6 of culture, collagenase and stromelysin were stimulated at a ratio of CCD18 fibroblasts to T24 cells of 1:0.1, while the maximum TIMP-1 production occurred at a ratio of 1:1. Thus, the cellular ratio in the interaction of carcinoma cells with host fibroblasts affects the production of TIMP-1 and metalloproteinases and hence modulates the balance between them.
巻・号 78(1-3)
ページ 133-40
公開日 1994-4-1
DOI 10.1016/0304-3835(94)90042-6
PII 0304-3835(94)90042-6
PMID 8180955
MeSH Cells, Cultured Culture Media Enzyme Induction Glycoproteins / biosynthesis* Humans Metalloendopeptidases / metabolism* Tissue Inhibitor of Metalloproteinases Tumor Cells, Cultured / enzymology*
IF 7.36
引用数 9
WOS 分野 ONCOLOGY
リソース情報
ヒト・動物細胞 T24(RCB0431)