RRC ID 38753
Author Takano M, Yokoyama K, Yayama K, Okamoto H.
Title Rat fibroblasts synthesize T-kininogen in response to cyclic-AMP, prostaglandin E2 and cytokines.
Journal Biochim Biophys Acta
Abstract T-Kininogen is a plasma protein characterized as a kinin-precursor, a cysteine protease inhibitor and an acute phase protein in the rat. Rat fibroblasts prepared from meninges or embryos and 3Y1-B clone 1-6 cells, a rat fibroblast cell line, secreted T-kininogen. Incubating these cells with 1 mM Bt2cAMP or a combination with 1 microM dexamethasone resulted in a marked increase in T-kininogen secretion, as well as in the incorporation of radioactive methionine into newly synthesized T-kininogen. Secretion of T-kininogen by meningeal fibroblasts was stimulated by forskolin, prostaglandin E2, bradykinin and cytokines, such as tumor necrosis factor alpha, interleukin-1 alpha (IL-1) and IL-6. Expression of T-kininogen mRNA was demonstrated in meningeal fibroblasts by Northern blot hybridization using T-kininogen cDNA as a probe, and the expression was stimulated by Bt2cAMP, prostaglandin E2, and the cytokines described above. In contrast, expression of T-kininogen mRNA in rat hepatocytes was not altered by Bt2cAMP, prostaglandin E2, tumor necrosis factor and IL-1, whereas it was greatly stimulated by IL-6, suggesting the differential regulation of T-kininogen gene expression in fibroblasts and hepatocytes. These results demonstrated for the first time, that rat fibroblasts express the T-kininogen gene, and that the expression is regulated by inflammatory mediators and cytokines.
Volume 1268(1)
Pages 107-14
Published 1995-7-20
DOI 10.1016/0167-4889(95)00048-w
PII 0167-4889(95)00048-W
PMID 7626656
MeSH Adenylyl Cyclases / agonists Animals Bucladesine / pharmacology* Cells, Cultured Colforsin / pharmacology Cytokines / pharmacology* Dexamethasone / pharmacology Dinoprostone / pharmacology* Fibroblasts / drug effects Fibroblasts / metabolism* Gene Expression Kininogens / biosynthesis* Kininogens / metabolism Liver / cytology Liver / metabolism Meninges / cytology Meninges / metabolism RNA, Messenger / biosynthesis Rats Rats, Sprague-Dawley
IF 3.411
Times Cited 12
Human and Animal Cells 3Y1-B clone 1-6(RCB0488)