| RRC ID |
38759
|
| 著者 |
Maruyama K, Tsukada T, Bandoh S, Sasaki K, Ohkura N, Yamaguchi K.
|
| タイトル |
Expression of NOR-1 and its closely related members of the steroid/thyroid hormone receptor superfamily in human neuroblastoma cell lines.
|
| ジャーナル |
Cancer Lett
|
| Abstract |
Previously, we isolated a cDNA of neuron derived orphan receptor (NOR-1), a putative transcription factor with strong homologies to the orphan nuclear receptors NGFI-B and NURR1. In the present study, we examined the gene expression of NOR-1 as well as NGFI-B and NURR1 in human neuroblastoma cell lines by reverse transcription-polymerase chain reaction and nucleotide sequencing. Although the mRNAs of these orphan receptors were detected in all six neuroblastoma cell lines examined, basal expression levels of these genes varied among cell lines. Treatment with forskolin and 12-O-tetradecanoylphorbol-13-acetate rapidly increased the expression of all these genes in neuroblastoma NB-OK-1 cells. This induction did not require de novo protein synthesis, indicating that the NOR-1 gene as well as NGFI-B and NURR1 genes is an immediate-early gene. This is the first demonstration of NOR-1 gene expression in tumor cell lines.
|
| 巻・号 |
96(1)
|
| ページ |
117-22
|
| 公開日 |
1995-9-4
|
| DOI |
10.1016/0304-3835(95)03921-i
|
| PII |
030438359503921I
|
| PMID |
7553599
|
| MeSH |
Base Sequence
Cell Differentiation / drug effects
Cell Line
Colforsin / pharmacology
Cycloheximide / pharmacology
DNA Primers / chemistry
DNA-Binding Proteins / genetics
Gene Expression
Humans
Molecular Sequence Data
Nerve Tissue Proteins / genetics
Neuroblastoma / genetics*
Nuclear Receptor Subfamily 4, Group A, Member 1
Nuclear Receptor Subfamily 4, Group A, Member 2
RNA, Messenger / genetics
RNA, Neoplasm / genetics
Receptors, Cytoplasmic and Nuclear
Receptors, Steroid / genetics*
Receptors, Thyroid Hormone / genetics*
Tetradecanoylphorbol Acetate / pharmacology
Transcription Factors / genetics
Tumor Cells, Cultured
|
| IF |
7.36
|
| 引用数 |
42
|
|
WOS 分野
|
ONCOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
LA-N-1(RCB0483)
LA-N-2(RCB0484)
LA-N-5(RCB0485) |
