論文 - 詳細
| RRC ID | 38815 |
|---|---|
| 著者 | Shin SY, Shimizu M, Ohtaki T, Munekata E. |
| タイトル | Synthesis and biological activity of N-terminal-truncated derivatives of human epidermal growth factor (h-EGF). |
| ジャーナル | Peptides |
| Abstract |
To investigate the contribution of the N-terminal sequence of h-EGF to its biological activity and the formation of three intramolecular disulfide bonds by oxidative refolding via air oxidation, five derivatives of h-EGF with a single N-terminal amino acid deletion were synthesized by solid-phase synthesis. The homogeneity of the synthetic peptides was confirmed by analytical reversed-phase HPLC, amino acid analysis, and FAB-MS. The pairing of the three disulfide bridges in synthetic peptides was determined by thermolytic digestion. All N-truncated derivatives of h-FGF formed the correct intramolecular three disulfide linkages during oxidative refolding and had equipotent activity in both EGF receptor binding on A-431 epidermoid carcinoma cells and mitogenesis on NIH-3T3 fibroblast cells, compared with authentic h-EGF. The results suggested that the five residues from N-terminal sequence of h-EGF have no effect on the formation of the correct disulfide linkages in h-EGF and do not exert a significant influence on its biological activity. |
| 巻・号 | 16(2) |
| ページ | 205-10 |
| 公開日 | 1995-1-1 |
| DOI | 10.1016/0196-9781(94)00181-2 |
| PII | 0196-9781(94)00181-2 |
| PMID | 7784250 |
| MeSH | 3T3 Cells Amino Acid Sequence Animals Binding, Competitive Carcinoma, Squamous Cell Cell Division / drug effects Chromatography, High Pressure Liquid Cysteine Dose-Response Relationship, Drug Epidermal Growth Factor / chemical synthesis* Epidermal Growth Factor / chemistry Epidermal Growth Factor / pharmacology* ErbB Receptors / metabolism* Humans Indicators and Reagents Kinetics Mice Molecular Sequence Data Peptide Fragments / chemical synthesis* Peptide Fragments / chemistry Peptide Fragments / pharmacology* Protein Conformation Protein Folding Tumor Cells, Cultured |
| IF | 2.843 |
| 引用数 | 7 |
| WOS 分野 | PHARMACOLOGY & PHARMACY BIOCHEMISTRY & MOLECULAR BIOLOGY ENDOCRINOLOGY & METABOLISM |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | A431(RCB0202) |